![Immunohistochemistry-Paraffin: B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free [NBP2-79932] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ICOSL-Monospecific Mouse Monoclonal Antibody (ICOSL/3260).](http://images.novusbio.com/fullsize/B7-H2-ICOSLG-Antibody-ICOSL-3260-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79932-img0001.jpg "Immunohistochemistry-Paraffin: B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free [NBP2-79932] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ICOSL-Monospecific Mouse Monoclonal Antibody (ICOSL/3260).")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, IHC, MA |
| Clone | ICOSL/3260 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79798). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Recombinant fragment of human B7-H2/ICOSLG protein (around aa 23-149) (exact sequence is proprietary) (Uniprot: O75144) |
| Localization | Cell Surface |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ICOSLG |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: For coating, order antibody without BSA). Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for B7-H2/ICOSLG Antibody (NBP2-79932)Find related products by research area.
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| Gene Symbol | ICOSLG |
![Protein Array: B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free [NBP2-79932] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.](http://images.novusbio.com/fullsize/B7-H2-ICOSLG-Antibody-ICOSL-3260-Azide-and-BSA-Free-Protein-Array-NBP2-79932-img0002.jpg "Protein Array: B7-H2/ICOSLG Antibody (ICOSL/3260) - Azide and BSA Free [NBP2-79932] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.")
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