Home » B7-H2/ICOSLG » B7-H2/ICOSLG Antibodies » B7-H2/ICOSLG Antibody (987614) [Unconjugated]
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B7-H2/ICOSLG Antibody (987614) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with either (A) mouse B7-H2 or (B) irrelevant transfectants and eGFP were stained with Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB1582) followed by Goat Anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with either (A) mouse B7-H2 or (B) irrelevant transfectants and eGFP were stained with Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB1582) followed by Goat ...read more
In a functional flow cytometry test, Recombinant Mouse ICOS Fc Chimera (168-CS; 50 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with mouse B7-H2. Binding is blocked by (A) 2.5 µg/mL of Rat ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, B/N, CyTOF-ready
Clone
987614
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1582U-100UG
Alexa Fluor 405
FAB1582V-100UG
Alexa Fluor 488
FAB1582G-100UG
Alexa Fluor 532
FAB1582X
Alexa Fluor 594
FAB1582T-100UG
Alexa Fluor 647
FAB1582R-100UG
Alexa Fluor 700
FAB1582N-100UG
Alexa Fluor 750
FAB1582S-100UG
Allophycocyanin
FAB1582A
Allophycocyanin/Cy7
FAB1582APCCY7
Biotin
FAB1582B
CoraFluor 1
FAB1582CL1
DyLight 350
FAB1582D
DyLight 405
FAB1582E
DyLight 488
FAB1582K
DyLight 550
FAB1582L
DyLight 594
FAB1582M
DyLight 650
FAB1582W
DyLight 680
FAB1582Y
DyLight 755
FAB1582Z
FITC
FAB1582F
HRP
FAB1582H
Janelia Fluor 525
FAB1582JF525
Janelia Fluor 549
FAB1582I
Janelia Fluor 585
FAB1582JF585
Janelia Fluor 635
FAB1582JF635
Janelia Fluor 646
FAB1582J
Janelia Fluor 669
FAB1582JF669
PE
FAB1582P
PE/Atto594
FAB1582PEATT594
PE/Cy5.5
FAB1582PECY55
PE/Cy7
FAB1582PECY7
PerCP
FAB1582C
mFluor Violet 450 SE
FAB1582MFV450
mFluor Violet 500 SE
FAB1582MFV500
mFluor Violet 610 SE
FAB1582MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1582-SP
Carrier Free
FAB1582V-100UG
Carrier Free
FAB1582U-100UG
Carrier Free
FAB1582T-100UG
Carrier Free
FAB1582S-100UG
Carrier Free
FAB1582R-100UG
Carrier Free
FAB1582N-100UG
Carrier Free
FAB1582G-100UG
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B7-H2/ICOSLG Antibody (987614) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse B7-H2
Met1-Lys279
Accession # Q9JHJ8
Specificity
Detects mouse B7-H2 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction 2.5 ug/mL
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-H2/ICOSLG Antibody (987614) [Unconjugated]

  • B7H2
  • B7-H2
  • B7H2B7 homolog 2
  • B7-H2B7-related protein 1
  • B7RP-1
  • B7RP1B7-like protein Gl50
  • B7RP-1LICOS
  • CD275 antigen
  • CD275
  • GL50
  • GL50transmembrane protein B7-H2 ICOS ligand
  • ICOSL
  • ICOS-L
  • ICOSLG
  • inducible T-cell co-stimulator ligand
  • KIAA0653ICOS ligand

Background

B7-H2, also called B7RP-1 and ICOSL, is a 60 kDa member of the B7 family of immune costimulatory proteins. It is expressed on resting B cells, dendritic cells, monocytes, alveolar epithelial cells, and placental trophoblasts. B7-H2 interactions with ICOS promote lymphocyte differentiation and activation. Within the ECD, mouse B7-H2 shares 49% and 70% aa sequence identity with human and rat B7-H2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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B7-H2/ICOSLG
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B7-H2/ICOSLG Antibody (987614) [Unconjugated]
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