![Western Blot: B7-1/CD80 Antibody (2E5) [NBP2-02905] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-2E5-Western-Blot-NBP2-02905-img0019.jpg "Western Blot: B7-1/CD80 Antibody (2E5) [NBP2-02905] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | OTI2E5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human CD80 (NP_005182) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD80 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 29.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-02905 | Applications | Species |
|---|---|---|
| Herrera M, Soderberg M, Sabirsh A et al. Inhibition of T-cell activation by CTLA4-Fc is sufficient to ameliorate proteinuric kidney disease Am. J. Physiol. Renal Physiol. 2016-07-20 [PMID: 27440778] |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
ICC | Human | 06/21/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for B7-1/CD80 Antibody (NBP2-02905)Find related products by research area.
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CD80: A co-stimulator of T cell activation CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (OTI2E5) [NBP2-02905] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-2E5-Immunocytochemistry-Immunofluorescence-NBP2-02905-img0010.jpg "Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (OTI2E5) [NBP2-02905] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80.")
![Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of paraffin-embedded Human prostate tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-2E5-Immunohistochemistry-Paraffin-NBP2-02905-img0018.jpg "Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of paraffin-embedded Human prostate tissue using anti-CD80 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-2E5-Immunohistochemistry-Paraffin-NBP2-02905-img0011.jpg "Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD80 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-2E5-Immunohistochemistry-Paraffin-NBP2-02905-img0012.jpg "Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD80 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-2E5-Immunohistochemistry-Paraffin-NBP2-02905-img0013.jpg "Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CD80 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of paraffin-embedded Human colon tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-2E5-Immunohistochemistry-Paraffin-NBP2-02905-img0015.jpg "Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of paraffin-embedded Human colon tissue using anti-CD80 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of paraffin-embedded Human liver tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-2E5-Immunohistochemistry-Paraffin-NBP2-02905-img0016.jpg "Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of paraffin-embedded Human liver tissue using anti-CD80 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of paraffin-embedded Human pancreas tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-2E5-Immunohistochemistry-Paraffin-NBP2-02905-img0017.jpg "Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (2E5) [NBP2-02905] - Staining of paraffin-embedded Human pancreas tissue using anti-CD80 mouse monoclonal antibody.")
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![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
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![N/A CD40 Ligand/TNFSF5 [HRP]](https://images.novusbio.com/images2/DATA_CD40_Ligand_DCDL40_ELISA_624.jpg)
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![Immunocytochemistry CD83 Antibody (HB15e) [Unconjugated]](https://images.novusbio.com/images2/CD83_MAB1774_Immunocytochemistry_6572.jpg)
