![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the PE/Cy5 conjugate of NBP1-43385. Staining of 3-day LPS activated C57Bl/6 splenocytes with 0.03 ug of PE-Cy5 Armenian Hamster IgG Isotype Control (open histogram) or 0.03 ug of Anti-Mouse CD80 (B7-1) PE-Cy5 (filled histogram). Total cells](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0007.jpg "Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the PE/Cy5 conjugate of NBP1-43385. Staining of 3-day LPS activated C57Bl/6 splenocytes with 0.03 ug of PE-Cy5 Armenian Hamster IgG Isotype Control (open histogram) or 0.03 ug of Anti-Mouse CD80 (B7-1) PE-Cy5 (filled histogram). Total cells")
| Reactivity | Hu, Mu, FeSpecies Glossary |
| Applications | Flow, IHC, IP |
| Clone | 16-10A1 |
| Clonality | Monoclonal |
| Host | Armenian Hamster |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 0.5 mg/ml |
| Immunogen | The immunogen for this antibody was CD80. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Armenian Hamster |
| Gene | CD80 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.2) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Protein A or G purified |
| Publication using NBP1-43385 | Applications | Species |
|---|---|---|
| Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (FLOW, Mouse) Details: Citation using the PE format of this antibody. |
FLOW | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for B7-1/CD80 Antibody (NBP1-43385)Find related products by research area.
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CD80: A co-stimulator of T cell activation CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CD80 |
![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD80 (B7-1) Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0001.jpg "Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD80 (B7-1) Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC. Total viable cells were used for analysis.")
![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the Biotin conjugate of NBP1-43385. Staining of 3-day LPS-stimulated C57BL/6 splenocytes with 0.06 ug of Armenian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 ug of Anti-Mouse CD80 (B7-1) Biotin (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0004.jpg "Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the Biotin conjugate of NBP1-43385. Staining of 3-day LPS-stimulated C57BL/6 splenocytes with 0.06 ug of Armenian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 ug of Anti-Mouse CD80 (B7-1) Biotin (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.")
![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the FITC conjugate of NBP1-43385. Staining of unstimulated (left) or 3-day LPS-stimulated (right) C57BL/6 splenocytes with 0.125 ug of Anti-Mouse CD80 (B7-1) FITC. Total viable cells were used for analysis. Markers were set based on the autofluorescence sample.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0005.jpg "Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the FITC conjugate of NBP1-43385. Staining of unstimulated (left) or 3-day LPS-stimulated (right) C57BL/6 splenocytes with 0.125 ug of Anti-Mouse CD80 (B7-1) FITC. Total viable cells were used for analysis. Markers were set based on the autofluorescence sample.")
![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the PE conjugate of NBP1-43385. See Notes](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0006.jpg "Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the PE conjugate of NBP1-43385. See Notes")
![Flow Cytometry: Armenian Hamster Monoclonal B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Alexa Fluor® 488 conjugated antibody (Catalog # NBP1-43385AF488) was tested via flow cytometry using magnetic beads coated with feline protein. Histogram overlay showing fluorescence results obtained via flow cytometry. Red line (unstained beads) and green line (beads coated with CD86 synthetic protein) served as negative controls. Blue line: positive signal obtained with CD80-coated beads. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-43385_armenian-hamster-monoclonal-b7-1-cd80-antibody-16-10a1-flow-cytometry-199202495917..png "Flow Cytometry: Armenian Hamster Monoclonal B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Alexa Fluor® 488 conjugated antibody (Catalog # NBP1-43385AF488) was tested via flow cytometry using magnetic beads coated with feline protein. Histogram overlay showing fluorescence results obtained via flow cytometry. Red line (unstained beads) and green line (beads coated with CD86 synthetic protein) served as negative controls. Blue line: positive signal obtained with CD80-coated beads. Image from a verified customer review.")
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