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ATP5J2 Antibody

Images

 
Western Blot: ATP5J2 Antibody [NBP2-57465] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunohistochemistry-Paraffin: ATP5J2 Antibody [NBP2-57465] - Staining of human kidney shows strong cytoplasmic positivity with a granular pattern in cells in tubules.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ATP5J2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LFSYSFSYKHLKHERLRKYH
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP5MF
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
ATP5J2 Recombinant Protein Antigen (NBP2-57465PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for ATP5J2 Antibody

  • ATP synthase f chain, mitochondrial
  • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit f
  • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit f, isoform 2
  • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2
  • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2
  • ATP5JLATP synthase subunit f, mitochondrial
  • F1F0-type ATPase subunit f
  • F1Fo-ATP synthase complex Fo membrane domain f subunit
  • F1Fo-ATPase synthase f subunit
  • F1Fo-ATPase

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the innermembrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalyticcore, F1, and the membrane-spanning component, F0, which comprises the proton channel. The catalytic portion ofmitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with astoichiometry of 3 alpha, 3 beta, and single representatives of the gamma, delta, and epsilon subunits. The protonchannel likely has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the f subunit of the F0 complex.Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This genehas multiple pseudogenes. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ATP5MF