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Protein Kinase D2 Antibody (OTI5G1)

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Western Blot: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein Kinase D2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Protein Kinase D2.
Western Blot: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein Kinase D2 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Protein Kinase D2.
Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Human Kidney tissue using anti-Protein Kinase D2 mouse monoclonal antibody.
Flow Cytometry: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Protein Kinase D2 ...read more
Western Blot: Protein Kinase D2 Antibody (5G1) [NBP2-02984] Analysis of extracts (35ug) from 9 different cell lines by using anti-Protein Kinase D2 monoclonal antibody.
Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Protein Kinase D2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Protein Kinase D2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Protein Kinase D2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI5G1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.72 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73664AF350
Alexa Fluor 405
NBP2-73664AF405
Alexa Fluor 488
NBP2-73664AF488
Alexa Fluor 532
NBP2-73664AF532
Alexa Fluor 594
NBP2-73664AF594
Alexa Fluor 647
NBP2-73664AF647
Alexa Fluor 700
NBP2-73664AF700
Alexa Fluor 750
NBP2-73664AF750
Allophycocyanin
NBP2-73664APC
Allophycocyanin/Cy7
NBP2-73664APCCY7
Biotin
NBP2-73664B
CoraFluor 1
NBP2-73664CL1
DyLight 350
NBP2-73664UV
DyLight 405
NBP2-73664V
DyLight 488
NBP2-73664G
DyLight 550
NBP2-73664R
DyLight 594
NBP2-73664DL594
DyLight 650
NBP2-73664C
DyLight 680
NBP2-73664FR
DyLight 755
NBP2-73664IR
FITC
NBP2-73664F
HRP
NBP2-73664H
Janelia Fluor 525
NBP2-73664JF525
Janelia Fluor 549
NBP2-73664JF549
Janelia Fluor 585
NBP2-73664JF585
Janelia Fluor 635
NBP2-73664JF635
Janelia Fluor 646
NBP2-73664JF646
Janelia Fluor 669
NBP2-73664JF669
PE
NBP2-73664PE
PE/Atto594
NBP2-73664PEATT594
PE/Cy5.5
NBP2-73664PECY55
PE/Cy7
NBP2-73664PECY7
PerCP
NBP2-73664PCP
mFluor Violet 450 SE
NBP2-73664MFV450
mFluor Violet 500 SE
NBP2-73664MFV500
mFluor Violet 610 SE
NBP2-73664MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-73664
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Protein Kinase D2 Antibody (OTI5G1) Summary

Immunogen
Full length human recombinant protein of human PRKD2 (NP_057541) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PRKD2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
96.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.72 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Protein Kinase D2 Antibody (OTI5G1)

  • DKFZP586E0820
  • EC 2.7.11
  • EC 2.7.11.13
  • nPKC-D2
  • PKD2HSPC187
  • PRKD2
  • protein kinase D2
  • serine/threonine-protein kinase D2

Background

The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PRKD2
Entrez