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ATF2 Antibody (3D12)

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Immunohistochemistry: ATF2 Antibody (3D12) [NBP3-26498] - Image of ATF2 Antibody (3D12) diluted at 1:115.5 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen ...read more
Western Blot: ATF2 Antibody (3D12) [NBP3-26498] - Positive Western Blot detected in: K562 whole cell lysate, 293T whole cell lysate. All lanes: ATF2 Antibody at 1.2ug/ml. Secondary: Goat polyclonal to rabbit IgG at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
3D12
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ATF2 Antibody (3D12) Summary

Additional Information
Recombinant monoclonal antibody expressed in HEK293F cells
Immunogen
A synthesized peptide derived from Human ATF2 [UniProt P15336]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ATF2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for ATF2 Antibody (3D12)

  • activating transcription factor 2formerly
  • ATF2
  • cAMP responsive element binding protein 2
  • cAMP-responsive element-binding protein 2
  • CREB2
  • CREBP1
  • CRE-BP1
  • cyclic AMP-dependent transcription factor ATF-2
  • HB16
  • MGC111558
  • mXBP
  • TREB7

Background

ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol ATF2