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Recombinant Human Angiopoietin-2 His Protein

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SDS-Page: Recombinant Human Angiopoietin-2 His Protein [NBP2-77507] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
SDS-Page: Recombinant Human Angiopoietin-2 His Protein [NBP2-77507] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Bioactivity: Recombinant Human Angiopoietin-2 His Protein [NBP2-77507] - Human Angiopoietin-2 is coated at 5 ug/ml (100 ul/well) and can bind Human Tie-2 in a Functional ELISA assay

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Concentration
0.25 mg/ml

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Recombinant Human Angiopoietin-2 His Protein Summary

Description
A recombinant bioactive protein with a C terminal His-tag and corresponding to the amino acids 19-496 of Human Angiopoietin-2.

Source: CHO cell

Amino Acid Sequence: YNNFRKSMDS IGKKQYQVQH GSCSYTFLLP EMDNCRSSSS PYVSNAVQRD APLEYDDSVQ RLQVLENIME NNTQWLMKLE NYIQDNMKKEMVEIQQNAVQ NQTAVMIEIG TNLLNQTAEQ TRKLTDVEAQ VLNQTTRLEL QLLEHSLSTN KLEKQILDQT SEINKLQDKN SFLEKKVLAMEDKHIIQLQS IKEEKDQLQV LVSKQNSIIE ELEKKIVTAT VNNSVLQKQQ HDLMETVNNL LTMMSTSNSA KDPTVAKEEQ ISFRDCAEVFKSGHTTNGIY TLTFPNSTEE IKAYCDMEAG GGGWTIIQRR EDGSVDFQRT WKEYKVGFGN PSGEYWLGNE FVSQLTNQQR YVLKIHLKDWEGNEAYSLYE HFYLSSEELN YRIHLKGLTG TAGKISSISQ PGNDFSTKDG DNDKCICKCS QMLTGGWWFD ACGPSNLNGM YYPQRQNTNKFNGIKWYYWK GSGYSLKATT MMIRPADFHH HHHH
Details of Functionality
Measured by its binding ability in a functional ELISA with Human Tie-2
Source
CHO
Protein/Peptide Type
Recombinant Protein
Gene
ANGPT2
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
55.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% glycerol.
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Angiopoietin-2 His Protein

  • AGPT2
  • ANG2
  • ANG-2
  • angiopoietin 2
  • Angiopoietin-2
  • angiopoietin-2a
  • angiopoietin-2B
  • angiopoitin 2
  • ANGPT2
  • Tie2-ligand

Background

Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2/Tek. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin 1 binding to Tie 2/Tek causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Human Angiopoietin-2 His Protein
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Bioinformatics

Gene Symbol ANGPT2