Name: Tie-2 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF313
Rating: 4 (1 reviews)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, Simple Western, IHC, B/N
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Biotin BAF313

Catalog# & Formulation	Size	Price
Carrier Free AF313-SP

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF313

Tie-2 Antibody [Unconjugated]

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Publications for Tie-2 Antibody (AF313)(44)

Review for Tie-2 Antibody (AF313) (1) 41

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Immunogen	Mouse myeloma cell line NS0-derived recombinant human Tie‑2 Ala23-Lys745 Accession # Q02763
Specificity	Detects human Tie-2 in direct ELISAs and Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	TEK
Purity Statement	Antigen Affinity-purified
Endotoxin Note	<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Publications using AF313	Applications	Species
Kuheli Banerjee, Yanzhu Lin, Johannes Gahn, Julio Cordero, Purnima Gupta, Islam Mohamed et al. SMAD4 maintains the fluid shear stress set point to protect against arterial-venous malformations Journal of Clinical Investigation 2023-09-15 [PMID: 37490341]
Ryoko Sato-Nishiuchi, Masamichi Doiguchi, Nanami Morooka, Kiyotoshi Sekiguchi Polydom/SVEP1 binds to Tie1 and promotes migration of lymphatic endothelial cells Journal of Cell Biology 2023-09-04 [PMID: 37338522]
Soo‐Been Jeon, A‐Reum Han, Sunghun Lee, Seung Chan Lee, Min Ji Lee, Soon‐Jung Park et al. CD34dim cells identified as pluripotent stem cell‐derived definitive hemogenic endothelium purified using bone morphogenetic protein 4 Cell Proliferation 2023-02-01 [PMID: 36478274]
Soo-Been Jeon, A-Reum Han, Yoo Bin Choi, Ah Reum Lee, Ji Yoon Lee Lymphoid Lineage gamma δ T Cells Were Successfully Generated from Human Pluripotent Stem Cells via Hemogenic Endothelium International Journal of Stem Cells 2023-02-28 [PMID: 36310028]
M Reijrink, J van Ark, CPH Lexis, LM Visser, ME Lodewijk, ICC van der Ho, CJ Zeebregts, H van Goor, SCA de Jager, G Pasterkamp, BHR Wolffenbut, JL Hillebrand Increased frequency of proangiogenic tunica intima endothelial kinase 2 (Tie2) expressing monocytes in individuals with type 2 diabetes mellitus Cardiovascular Diabetology, 2022-05-12;21(1):72. 2022-05-12 [PMID: 35549955] (IHC, Human)	IHC	Human
LT Hsieh, SJ Dos Santos, BS Hall, J Ogbechi, AD Loglo, FJ Salguero, MT Ruf, G Pluschke, RE Simmonds Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1beta, are linked to fibrin formation in Buruli ulcer lesions PloS Pathogens, 2022-01-31;18(1):e1010280. 2022-01-31 [PMID: 35100311] (Western Blot, Human)	Western Blot	Human
G Jo, J Bae, HJ Hong, AR Han, DK Kim, SP Hong, JA Kim, S Lee, GY Koh, HM Kim Structural insights into the clustering and activation of Tie2 receptor mediated by Tie2 agonistic antibody Nature Communications, 2021-11-01;12(1):6287. 11/1/2021 [PMID: 34725372]
M Adjuto-Sac, P Soubeyran, J Garcia, S Audebert, L Camoin, M Rubis, J Roques, B Binétruy, JL Iovanna, R Tournaire TNF-&alpha induces endothelial-mesenchymal transition promoting stromal development of pancreatic adenocarcinoma Cell Death & Disease, 2021-06-25;12(7):649. 2021-06-25 [PMID: 34172716] (Immunoprecipitation, Human)	Immunoprecipitation	Human
Erica Villa, Rosina Critelli, Simone Lasagni, Alessandra Melegari, Angela Curatolo, Ciro Celsa et al. Dynamic angiopoietin-2 assessment predicts survival and chronic course in hospitalized patients with COVID-19 Blood Advances 2021-02-09 [PMID: 33560382]
F Heil, G Babitzki, A Julien-Laf, CH Ooi, M Hidalgo, C Massard, M Martinez-G, C Le Tournea, M Kockx, P Gerber, S Rossomanno, O Krieter, A Lahr, N Wild, SV Harring, K Lechner Vanucizumab mode of action: Serial biomarkers in plasma, tumor, and skin-wound-healing biopsies Translational Oncology, 2020-12-15;14(2):100984. 2020-12-15 [PMID: 33338877] (IHC, Human)	IHC	Human
ME Griffin, AW Sorum, GM Miller, WA Goddard, LC Hsieh-Wils Sulfated glycans engage the Ang-Tie pathway to regulate vascular development Nat Chem Biol, 2020-10-05;0(0):. 2020-10-05 [PMID: 33020664] (Western Blot, Human)	Western Blot	Human
Q Han, Q Zhang, F Ying, Z Wang, Y Zhang, L Gong, E Cai, J Qian, J Cai Circulating Tie2-Expressing Monocytes: A Potential Biomarker for Cervical Cancer OncoTargets and Therapy, 2020-09-07;13(0):8877-8885. 9/7/2020 [PMID: 32982281]
A Chatterjee, R Eshwaran, H Huang, D Zhao, M Schmidt, T Wieland, Y Feng Role of the Ang2-Tie2 Axis in Vascular Damage Driven by High Glucose or Nucleoside Diphosphate Kinase B Deficiency Int J Mol Sci, 2020-05-25;21(10):. 2020-05-25 [PMID: 32466219] (Immunoprecipitation, Human)	Immunoprecipitation	Human
J Gu, Y Zhang, Z Han, L Gao, J Cui, Y Sun, Y Niu, B You, CP Huang, C Chang, X Wang, S Yeh Targeting the ER&beta/Angiopoietin-2/Tie-2 signaling-mediated angiogenesis with the FDA-approved anti-estrogen Faslodex to increase the Sunitinib sensitivity in RCC Cell Death Dis, 2020-05-14;11(5):367. 2020-05-14 [PMID: 32409702] (Western Blot, Human)	Western Blot	Human
Mélissande Cossutta, Marie Darche, Gilles Carpentier, Claire Houppe, Matteo Ponzo, Fabio Raineri et al. Weibel-Palade Bodies Orchestrate Pericytes During Angiogenesis Arteriosclerosis, Thrombosis, and Vascular Biology 2019-09-01 [PMID: 31315435]
Marie Besnier, Saran Shantikumar, Maryam Anwar, Parul Dixit, Aranzazu Chamorro-Jorganes, Walid Sweaad et al. miR-15a/-16 Inhibit Angiogenesis by Targeting the Tie2 Coding Sequence: Therapeutic Potential of a miR-15a/16 Decoy System in Limb Ischemia Molecular Therapy - Nucleic Acids 2019-09-06 [PMID: 31220779]
J Kim, JR Park, J Choi, I Park, Y Hwang, H Bae, Y Kim, W Choi, JM Yang, S Han, TY Chung, P Kim, Y Kubota, HG Augustin, WY Oh, GY Koh Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration Sci Adv, 2019-02-13;5(2):eaau6732. 2019-02-13 [PMID: 30788433] (ICC, IHC-Fr, Immunoprecipitation, Human, Mouse)	ICC, IHC-Fr, Immunoprecipitation	Human, Mouse
T Tsujimoto, H Sudo, M Todoh, K Yamada, K Iwasaki, T Ohnishi, N Hirohama, T Nonoyama, D Ukeba, K Ura, YM Ito, N Iwasaki An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair: A preclinical proof-of-concept study EBioMedicine, 2018-10-30;0(0):. 2018-10-30 [PMID: 30389504] (ICC, Rabbit)	ICC	Rabbit
VM Leppänen, P Saharinen, K Alitalo Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization Proc. Natl. Acad. Sci. U.S.A., 2017-04-10;0(0):. 2017-04-10 [PMID: 28396439] (Western Blot, Human)	Western Blot	Human
Sampedro-Nunez M, Luque RM, Ramos-Levi AM et al. Presence of sst5TMD4, a truncated splice variant of the somatostatin receptor subtype 5, is associated to features of increased aggressiveness in pancreatic neuroendocrine tumors. Oncotarget [PMID: 26673010] Details: Used the Alexa Fluor 488 form of this antibody.
Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P Endothelial destabilization by angiopoietin-2 via integrin beta1 activation. Nat Commun, 2015-01-30;6(0):5962. 2015-01-30 [PMID: 25635707] (ICC, IHC, Human)	ICC, IHC	Human
Samuel Martín-Vílchez, Yolanda Rodríguez-Muñoz, Rosario López-Rodríguez, Ángel Hernández-Bartolomé, María Jesús Borque-Iñurrita, Francisca Molina-Jiménez et al. Inhibition of Tyrosine Kinase Receptor Tie2 Reverts HCV-Induced Hepatic Stellate Cell Activation PLoS ONE 2014-10-10 [PMID: 25302785]
Vermi W, Micheletti A, Lonardi S, Costantini C, Calzetti F, Nascimbeni R, Bugatti M, Codazzi M, Pinter P, Schakel K, Tamassia N, Cassatella M slanDCs selectively accumulate in carcinoma-draining lymph nodes and marginate metastatic cells. Nat Commun, 2014-01-01;5(0):3029. 2014-01-01 [PMID: 24398631] (Western Blot, Human)	Western Blot	Human
Sakai , Daisuke, Nakamura , Yoshihik, Nakai , Tomoko, Mishima , Taishi, Kato , Shunichi, Grad , Sibylle, Alini , Mauro, Risbud , Makarand, Chan , Danny, Cheah , Kathryn, Yamamura , Ken-ichi, Masuda , Koichi, Okano , Hideyuki, Ando , Kiyoshi, Mochida , Joji Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc. Nat Commun, 2012-01-01;3(0):1264. 2012-01-01 [PMID: 23232394] (Neutralization, Human)	Neutralization	Human
Jimmy Masjkur, Maria Adele Adele Rueger, Stefan R R. Bornstein, Ron McKay, Andreas Androutsellis-Theotokis Neurovascular Signals Suggest a Propagation Mechanism for Endogenous Stem Cell Activation Along Blood Vessels CNS & Neurological Disorders - Drug Targets 2012-12-01 [PMID: 23131162]
van Rensburg , R, Beyer , I, Yao , X-Y, Wang , H, Denisenko , O, Li , Z-Y, Russell , D W, Miller , D G, Gregory , P, Holmes , M, Bomsztyk , K, Lieber , A Chromatin structure of two genomic sites for targeted transgene integration in induced pluripotent stem cells and hematopoietic stem cells. Gene Ther, 2012-03-22;20(2):201-14. 2012-03-22 [PMID: 22436965] (ICC, Human)	ICC	Human
van der Heijden M, van Nieuw Amerongen GP, van Bezu J, Paul MA, Groeneveld AB, van Hinsbergh VW Opposing effects of the angiopoietins on the thrombin-induced permeability of human pulmonary microvascular endothelial cells. PLoS ONE, 2011-08-15;6(8):e23448. 2011-08-15 [PMID: 21858121] (Immunoprecipitation, Western Blot, Human)	Immunoprecipitation, Western Blot	Human
Ricci-Vitiani L, Pallini R, Biffoni M, Todaro M, Invernici G, Cenci T, Maira G, Parati EA, Stassi G, Larocca LM, De Maria R Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells. Nature, 2010-11-21;468(7325):824-8. 2010-11-21 [PMID: 21102434] (Flow Cytometry, IHC-P, Human)	Flow Cytometry, IHC-P	Human
Roy H, Bhardwaj S, Babu M, Lahteenvuo JE, Yla-Herttuala S VEGF-DdeltaNdeltaC mediated angiogenesis in skeletal muscles of diabetic WHHL rabbits. Eur. J. Clin. Invest., 2010-05-01;40(5):422-32. 2010-05-01 [PMID: 20534064] (IHC-P, Rabbit)	IHC-P	Rabbit
Andreas Androutsellis-Theotokis, Stuart Walbridge, Deric M. Park, Russell R. Lonser, Ronald D. G. McKay Cholera Toxin Regulates a Signaling Pathway Critical for the Expansion of Neural Stem Cell Cultures from the Fetal and Adult Rodent Brains PLoS ONE 2010-05-26 [PMID: 20520777]
Sung-Suk Chae, Walid S. Kamoun, Christian T. Farrar, Nathaniel D. Kirkpatrick, Elisabeth Niemeyer, Annemarie M.A. de Graaf et al. Angiopoietin-2 interferes with anti-VEGFR2- induced vessel normalization and survival benefit in mice bearing gliomas Clinical Cancer Research 2010-07-15 [PMID: 20501615]
Rosa AI, Goncalves J, Cortes L, Bernardino L, Malva JO, Agasse F The angiogenic factor angiopoietin-1 is a proneurogenic peptide on subventricular zone stem/progenitor cells. J. Neurosci., 2010-03-31;30(13):4573-84. 2010-03-31 [PMID: 20357108] (ICC, IHC, Neutralization, Western Blot, Mouse)	ICC, IHC, Neutralization, Western Blot	Mouse
Ramazan Demir Expression of VEGF receptors VEFGR-1 and VEGFR-2, angiopoietin receptors Tie-1 and Tie-2 in chorionic villi tree during early pregnancy Folia Histochemica et Cytobiologica 2010-02-19 [PMID: 20164029]
Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation. Eur. J. Haematol., 2009-11-17;84(3):239-51. 2009-11-17 [PMID: 19922462] (Neutralization, Human)	Neutralization	Human
Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells. Exp. Hematol., 2008-06-17;36(9):1121-31. 2008-06-17 [PMID: 18562079] (Neutralization, Human)	Neutralization	Human
Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, Nottebaum A, Vestweber D, Deutsch U, Koh GY, Olsen BR, Alitalo K Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts. Nat. Cell Biol., 2008-04-20;10(5):527-37. 2008-04-20 [PMID: 18425119] (ICC, Western Blot, Human)	ICC, Western Blot	Human
Marron MB, Singh H, Tahir TA, Kavumkal J, Kim HZ, Koh GY, Brindle NP Regulated proteolytic processing of Tie1 modulates ligand responsiveness of the receptor-tyrosine kinase Tie2. J. Biol. Chem., 2007-08-29;282(42):30509-17. 2007-08-29 [PMID: 17728252] (Western Blot, Human)	Western Blot	Human
Kim KL, Shin IS, Kim JM, Choi JH, Byun J, Jeon ES, Suh W, Kim DK Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells. Cardiovasc. Res., 2006-08-07;72(3):394-402. 2006-08-07 [PMID: 17054925] (Immunoprecipitation, Neutralization, Western Blot, Human)	Immunoprecipitation, Neutralization, Western Blot	Human
Asikainen TM, Waleh NS, Schneider BK, Clyman RI, White CW Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo. Am. J. Physiol. Lung Cell Mol. Physiol., 2006-05-05;291(4):L588-95. 2006-05-05 [PMID: 16679381] (Western Blot, Primate - Papio anubis (Olive Baboon))	Western Blot	Primate - Papio anubis (Olive Baboon)
Kayisli UA, Cayli S, Seval Y, Tertemiz F, Huppertz B, Demir R Spatial and temporal distribution of Tie-1 and Tie-2 during very early development of the human placenta. Placenta, 2005-07-18;27(6):648-59. 2005-07-18 [PMID: 16026828] (IHC-P, Western Blot, Human)	IHC-P, Western Blot	Human
Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung. Proc. Natl. Acad. Sci. U.S.A., 2005-07-11;102(29):10212-7. 2005-07-11 [PMID: 16009933] (Western Blot, Human)	Western Blot	Human
Morris PN, Dunmore BJ, Tadros A, Marchuk DA, Darland DC, D&apos;Amore PA, Brindle NP Functional analysis of a mutant form of the receptor tyrosine kinase Tie2 causing venous malformations. J. Mol. Med., 2004-10-29;83(1):58-63. 2004-10-29 [PMID: 15526080] (ICC, IHC, Western Blot, Human)	ICC, IHC, Western Blot	Human
Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis. Blood, 2003-03-20;102(2):638-45. 2003-03-20 [PMID: 12649156] (Neutralization, Human)	Neutralization	Human
Marron MB, Hughes DP, Edge MD, Forder CL, Brindle NP Evidence for heterotypic interaction between the receptor tyrosine kinases TIE-1 and TIE-2. J. Biol. Chem., 2000-12-15;275(50):39741-6. 2000-12-15 [PMID: 10995770] (Immunoprecipitation, Human)	Immunoprecipitation	Human
Show All 44 Publications. Collapse Publications.

	Verified Customer 01/07/2015
Application:	IF
Species:	Human

Gene Symbol	TEK
Uniprot	Q02763()