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Angiopoietin-2 Antibody

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Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse Angiopoietin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7186) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Angiopoietin-2 at approximately 60 to 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse Angiopoietin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7186) followed by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF7186-SP
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Angiopoietin-2 Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Angiopoietin-2
Asp68-Phe496
Accession # O35608
Specificity
Detects mouse Angiopoietin-2 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human Angiopoietin-2, recombinant mouse (rm) Angiopoietin-1, and rmAngiopoietin-3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ANGPT2
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
Publications
Read Publications using
AF7186 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-2 Antibody

  • AGPT2
  • ANG2
  • ANG-2
  • angiopoietin 2
  • Angiopoietin-2
  • angiopoietin-2a
  • angiopoietin-2B
  • angiopoitin 2
  • ANGPT2
  • Tie2-ligand

Background

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted 66-70 kDa glycoprotein member of the angiopoietin family of growth factors. It is expressed by endothelial cells, macrophages and skeletal muscle fibers, and binds to the Tie2 receptor on select cell types, including endothelial cells and monocytes. Ang-2 has multiple functions. In the presence of growth factors, it promotes angiogenesis; in the absence of growth factors, it induces vascular regression. It is generally considered to be an antagonist of Ang-1 activity. Mature mouse Ang-2 is 478 amino acids (aa) in length (aa 19-496). It contains one coiled-coil region (aa 159-256) plus a C-terminal Fibrinogen-like domain (aa 275-495). Mouse Ang-2 undergoes covalent oligomerization, forming 140 kDa homodimers and 280 kDa homotetramers. The higher-order oligomers are strong Tie-2 agonists. Over aa 68-496, mouse Ang-2 shares 87% and 96% aa sequence identity with human and rat Ang-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Angiopoietin-2
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AF7186
Angiopoietin-2 Antibody
Species: Mu
Applications: WB

Publications for Angiopoietin-2 Antibody (AF7186)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, Western Blot.


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(1)
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Bioinformatics

Gene Symbol ANGPT2
Uniprot