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Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

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 (2 Reviews)
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Immunocytochemistry/Immunofluorescence: Angiopoietin-2 Antibody (MM0020-1F29) [NB110-85467] - Staining of frozen human skin sections using Angiopoietin 2 antibody at 1:25 dilution. Image provided via product review by verified customer.
Immunocytochemistry/ Immunofluorescence: Angiopoietin-2 Antibody (MM0020-1F29) [NB110-85467] - Staining of frozen human skin sections using Angiopoietin 2 antibody at 1:25 dilution. Image provided via product review by ...read more
Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) [NB110-85467] - Ang-2 (green) was detected in human skin (nevus) using Ang2-Alexa Fluor 488 antibody (1:40) in PBS for 1 hour. Nuclei were stained with DAPI ...read more
Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) [NB110-85467] - Normal Human placenta tissue

Product Details

Summary
Reactivity Hu, ChSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
MM0020-1F29
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-85467AF350
Alexa Fluor 405
NB110-85467AF405
Alexa Fluor 488
NB110-85467AF488
Alexa Fluor 532
NB110-85467AF532
Alexa Fluor 594
NB110-85467AF594
Alexa Fluor 647
NB110-85467AF647
Alexa Fluor 700
NB110-85467AF700
Alexa Fluor 750
NB110-85467AF750
Allophycocyanin
NB110-85467APC
Biotin
NB110-85467B
CoraFluor 1
NB110-85467CL1
DyLight 350
NB110-85467UV
DyLight 405
NB110-85467V
DyLight 488
NB110-85467G
DyLight 550
NB110-85467R
DyLight 594
NB110-85467DL594
DyLight 650
NB110-85467C
DyLight 680
NB110-85467FR
DyLight 755
NB110-85467IR
FITC
NB110-85467F
HRP
NB110-85467H
Janelia Fluor 525
NB110-85467JF525
Janelia Fluor 549
NB110-85467JF549
Janelia Fluor 646
NB110-85467JF646
Janelia Fluor 669
NB110-85467JF669
PE
NB110-85467PE
PerCP
NB110-85467PCP
mFluor Violet 450 SE
NB110-85467MFV450
mFluor Violet 500 SE
NB110-85467MFV500
mFluor Violet 610 SE
NB110-85467MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NB110-85467-0.025mg
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Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free Summary

Immunogen
Human recombinant Angiopoietin-2
Specificity
No cross reactivity with human Angiopoietin-1
Isotype
IgG2
Clonality
Monoclonal
Host
Mouse
Gene
ANGPT2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:25
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1:500-1:1000
Application Notes
By Western blot, this antibody detects a band at ~60-75 kDa under reducing conditions. Has been reported to work in Immunohistochemistry-Frozen (PMID 22302773) and Immunofluorescence in a customer review.
Control
Angiopoietin-2 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 3
using
NB110-85467 in the following applications:
Publications
Read Publications using
NB110-85467 in the following applications:

Reactivity Notes

Chicken data from customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

  • AGPT2
  • ANG2
  • ANG-2
  • angiopoietin 2
  • Angiopoietin-2
  • angiopoietin-2a
  • angiopoietin-2B
  • angiopoitin 2
  • ANGPT2
  • Tie2-ligand

Background

Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2/Tek. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin 1 binding to Tie 2/Tek causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Angiopoietin-2
Antibody (MM0020-1F29 ...
NB110-85467
Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free
Species: Hu, Ch
Applications: WB, ICC/IF, IHC

Publications for Angiopoietin-2 Antibody (NB110-85467)(5)

We have publications tested in 2 confirmed species: Human, Chicken.

We have publications tested in 2 applications: IF, IHC-Fr.


Filter By Application
IF
(1)
IHC-Fr
(1)
All Applications
Filter By Species
Human
(1)
Chicken
(1)
All Species

Reviews for Angiopoietin-2 Antibody (NB110-85467) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using NB110-85467:
Filter by Applications
IF
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Angiopoietin-2 NB110-85467
Enlarge 		3
reviewed by:
Bryan Tinsley
 IF Other 08/26/2014
View

Summary

ApplicationImmunofluorescence
SpeciesOther
Immunohistochemistry-Frozen Angiopoietin-2 NB110-85467
Enlarge 		3
reviewed by:
Corey .
 IHC-Fr Human 07/12/2013
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen human skin sections
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Angiopoietin-2 Antibody (NB110-85467). (Showing 1 - 2 of 2 FAQs).

  1. How do you dissolve the lyophilized Ab? Water or PBS? And in what concentration?
    • The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). 100 ug antibody is lyophilized from 40 ul of 1 x PBS. We suggest reconstituting the antibody with 500 ul sterile 1 x PBS (or to be perfect, with 40 ul H2O plus 460 ul PBS), to give a final concentration of 200 ug/ml. Alternatively, you may reconstitute the antibody in 40 ul H2O if a small volume is preferred.
  2. I have a question regarding your Angiopoietin 2 Antibody (MM0020-1F29). I am interested in differentiating Ang2 from mouse origin and Ang2 from human origin. Does this antibody cross react in any way with mouse Ang2?
    • This particular product has only been tested against human samples. We have not yet tested for cross reactivity with other species. Based on the homology of the full length protein, I would suspect that there may be some cross reaction. I located the amino acid range for this product. It is between aa70-aa496 of recombinant human Angiopoietin-2 (O15123). If you would be interested in testing this novel species, please take a look at our Innovator's Reward program.

Control Lysate(s)

Angiopoietin-2 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Research Areas for Angiopoietin-2 Antibody (NB110-85467)

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Recent Reviews

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5
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2
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1
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Immunofluorescence Angiopoietin-2 NB110-85467
Enlarge
 Bryan Tinsley
08/26/2014
Application: IF
Species: Other
read this review
Immunohistochemistry-Frozen Angiopoietin-2 NB110-85467
Enlarge
 Corey .
07/12/2013
Application: IHC-Fr
Species: Human
read this review

Bioinformatics

Gene Symbol ANGPT2
Entrez
Uniprot