Reactivity | Hu, RtSpecies Glossary |
Applications | Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
Clone | 1A4 + ACTA2/791 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Alexa Fluor 750 |
Immunogen | N-Terminal decapeptide of alpha-Smooth Muscle Actin and conjugated to KLH (1A4); Recombinant full-length human ACTA2 protein (ACTA2/791) (Uniprot: P62736) |
Localization | Cytoplasmic |
Marker | Leiomyosarcoma Marker |
Specificity | Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This monoclonal antibody does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin. |
Isotype | IgG2a Kappa/IgG2a Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | ACTA2 |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Optimal dilution of this antibody should be experimentally determined. |
Storage | Store at 4C in the dark. |
Buffer | 50mM Sodium Borate |
Preservative | 0.05% Sodium Azide |
Purity | Protein A or G purified |
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Gene Symbol | ACTA2 |