![Knockout Validated: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692] - Western blot shows lysates of HepG2 human liver carcinoma cells parental cell line and AFP knockout (KO) HepG2 cell line. PVDF membrane was probed with 0.05 ug/mL of Mouse Anti-Human AFP Monoclonal Antibody (Catalog # MAB13692) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for AFP at approximately 74 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in the knockout HepG2 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-189508-Knockout-Validated-MAB13692-img0005.jpg "Knockout Validated: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692] - Western blot shows lysates of HepG2 human liver carcinoma cells parental cell line and AFP knockout (KO) HepG2 cell line. PVDF membrane was probed with 0.05 ug/mL of Mouse Anti-Human AFP Monoclonal Antibody (Catalog # MAB13692) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for AFP at approximately 74 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in the knockout HepG2 cell line. This experiment was conducted under reducing conditions.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Simple Western, KO |
| Clone | 189508 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Human alpha-Fetoprotein MAb (Clone 189508) |
| Immunogen | Human umbilical cord serum-derived alpha -Fetoprotein/AFP |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | AFP |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A or G purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for alpha-Fetoprotein/AFP Antibody (MAB13692)Find related products by research area.
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| Gene Symbol | AFP |
![Western Blot: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692] - Western blot shows lysates of undifferentiated and differentiated IBJ6 human induced pluripotent stem cells, and HepG2 and Hep3B cell lines. PVDF membrane was probed with 0.05 ug/mL of Mouse Anti-Human AFP Monoclonal Antibody (Catalog # MAB13692) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for AFP in the differentiated IBJ6 cells at approximately 74 kDa (as indicated), the HepG2 cells and Hep3B cells. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-189508-Western-Blot-MAB13692-img0003.jpg "Western Blot: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692] - Western blot shows lysates of undifferentiated and differentiated IBJ6 human induced pluripotent stem cells, and HepG2 and Hep3B cell lines. PVDF membrane was probed with 0.05 ug/mL of Mouse Anti-Human AFP Monoclonal Antibody (Catalog # MAB13692) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for AFP in the differentiated IBJ6 cells at approximately 74 kDa (as indicated), the HepG2 cells and Hep3B cells. This experiment was conducted under reducing conditions.")
![Simple Western: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692] - Lane view shows a specific band for Fetoprotein-AFP in 0.2 mg/mL of HepG2 and HepB3 lysate. Antibody at 5 ug/mL. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-189508-Simple-Western-MAB13692-img0007.jpg "Simple Western: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692] - Lane view shows a specific band for Fetoprotein-AFP in 0.2 mg/mL of HepG2 and HepB3 lysate. Antibody at 5 ug/mL. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")