![Western Blot: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Analysis using ADD1 mAb against human ADD1 (AA: 1-193)recombinant protein. (Expected MW is 47.8 kDa)](http://images.novusbio.com/fullsize/alpha-Adducin-Antibody-5D4H1-Western-Blot-NBP2-52409-img0002.jpg "Western Blot: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Analysis using ADD1 mAb against human ADD1 (AA: 1-193)recombinant protein. (Expected MW is 47.8 kDa)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, CyTOF-ready |
| Clone | 5D4H1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human alpha Adducin (AA: 1-193) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ADD1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 81 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Immunohistochemistry ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Immunohistochemistry_6703.jpg)
![Western Blot ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Flow_Cytometry_20028.jpg)
![Simple Western ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Simple_Western_20109.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ADD1 |
![Immunohistochemistry-Paraffin: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Analysis of paraffin-embedded cervical cancer tissues using alpha Adducin mAb with DAB staining.](http://images.novusbio.com/fullsize/alpha-Adducin-Antibody-5D4H1-Immunohistochemistry-Paraffin-NBP2-52409-img0006.jpg "Immunohistochemistry-Paraffin: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Analysis of paraffin-embedded cervical cancer tissues using alpha Adducin mAb with DAB staining.")
![Western Blot: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Analysis using ADD1 mAb against HEK293 (1) and ADD1 (AA: 1-193)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/alpha-Adducin-Antibody-5D4H1-Western-Blot-NBP2-52409-img0003.jpg "Western Blot: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Analysis using ADD1 mAb against HEK293 (1) and ADD1 (AA: 1-193)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using 1-193 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/alpha-Adducin-Antibody-5D4H1-Immunohistochemistry-Paraffin-NBP2-52409-img0004.jpg "Immunohistochemistry-Paraffin: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using 1-193 mouse mAb with DAB staining.")
![ELISA: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/alpha-Adducin-Antibody-5D4H1-ELISA-NBP2-52409-img0005.jpg "ELISA: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Immunohistochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunohistochemistry_21186.jpg)
![Western Blot Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Western_Blot_11656.jpg)
![Immunocytochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunocytochemistry_11911.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")