![Immunocytochemistry/Immunofluorescence: AFG3L2 Antibody [NBP1-87086] - Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.](http://images.novusbio.com/fullsize/AFG3L2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87086-img0007.jpg "Immunocytochemistry/Immunofluorescence: AFG3L2 Antibody [NBP1-87086] - Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: KTLLAKATAGEANVPFITVSGSEFLEMFVGVGPARVRDLFALARKNAPCILFIDEIDAVGRKRGRGNFGGQSEQENTLNQLLVEMDGFNTTTNVVILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHLRPLK |
| Predicted Species | Mouse (100%), Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | AFG3L2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval method is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | AFG3L2 |
![Immunohistochemistry-Paraffin: AFG3L2 Antibody [NBP1-87086] - Staining of human liver.](http://images.novusbio.com/fullsize/AFG3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-87086-img0011.jpg "Immunohistochemistry-Paraffin: AFG3L2 Antibody [NBP1-87086] - Staining of human liver.")
![Immunohistochemistry-Paraffin: AFG3L2 Antibody [NBP1-87086] - Staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/AFG3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-87086-img0006.jpg "Immunohistochemistry-Paraffin: AFG3L2 Antibody [NBP1-87086] - Staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: AFG3L2 Antibody [NBP1-87086] - Staining of human colon, kidney, liver and testis using Anti-AFG3L2 antibody NBP1-87086 (A) shows similar protein distribution across tissues to independent antibody NBP1-87085 (B).](http://images.novusbio.com/fullsize/AFG3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-87086-img0008.jpg "Immunohistochemistry-Paraffin: AFG3L2 Antibody [NBP1-87086] - Staining of human colon, kidney, liver and testis using Anti-AFG3L2 antibody NBP1-87086 (A) shows similar protein distribution across tissues to independent antibody NBP1-87085 (B).")
![Immunohistochemistry-Paraffin: AFG3L2 Antibody [NBP1-87086] - Staining of human kidney.](http://images.novusbio.com/fullsize/AFG3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-87086-img0009.jpg "Immunohistochemistry-Paraffin: AFG3L2 Antibody [NBP1-87086] - Staining of human kidney.")
![Immunohistochemistry-Paraffin: AFG3L2 Antibody [NBP1-87086] - Staining of human colon.](http://images.novusbio.com/fullsize/AFG3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-87086-img0010.jpg "Immunohistochemistry-Paraffin: AFG3L2 Antibody [NBP1-87086] - Staining of human colon.")
![Immunohistochemistry-Paraffin: AFG3L2 Antibody [NBP1-87086] - Staining of human testis.](http://images.novusbio.com/fullsize/AFG3L2-Antibody-Immunohistochemistry-Paraffin-NBP1-87086-img0012.jpg "Immunohistochemistry-Paraffin: AFG3L2 Antibody [NBP1-87086] - Staining of human testis.")
![Immunohistochemistry-Paraffin TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-Paraffin-NB600-607-img0005.jpg)
![Western Blot TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0006.jpg)
![Data TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-N-A-NB600-607-img0008.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")