![Western Blot: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Western Blot Analysis of PANC-1 cell lysate using Spectrin beta 3 Antibody (SPTBN2/1584).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Azide-and-BSA-Free-Western-Blot-NBP2-54439-img0008.jpg "Western Blot: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Western Blot Analysis of PANC-1 cell lysate using Spectrin beta 3 Antibody (SPTBN2/1584).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, MA, CyTOF-ready |
| Clone | SPTBN2/1584 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53315). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant fragment (around aa356-475) of human Spectrin beta 3 protein (exact sequence is proprietary) (Uniprot: O15020) |
| Localization | Cell Surface |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SPTBN2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 246 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Spectrin beta 3 Antibody (NBP2-54439)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SPTBN2 |
![Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Immunofluorescence Analysis of MeOH-fixed HeLa cells labeling Spectrin beta III with Spectrin beta 3 Antibody (SPTBN2/1584) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54439-img0006.jpg "Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Immunofluorescence Analysis of MeOH-fixed HeLa cells labeling Spectrin beta III with Spectrin beta 3 Antibody (SPTBN2/1584) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).")
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Formalin-fixed, paraffin-embedded Mouse Kidney stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54439-img0002.jpg "Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Formalin-fixed, paraffin-embedded Mouse Kidney stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).")
![Flow Cytometry: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Flow Cytometric Analysis of HeLa cells using Spectrin beta 3 Antibody (SPTBN2/1584) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54439-img0004.jpg "Flow Cytometry: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Flow Cytometric Analysis of HeLa cells using Spectrin beta 3 Antibody (SPTBN2/1584) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Western Blot: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Western Blot Analysis of HeLa cell lysate using Spectrin beta 3 Antibody (SPTBN2/1584).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Azide-and-BSA-Free-Western-Blot-NBP2-54439-img0007.jpg "Western Blot: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Western Blot Analysis of HeLa cell lysate using Spectrin beta 3 Antibody (SPTBN2/1584).")
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54439-img0001.jpg "Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).")
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54439-img0003.jpg "Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).")
![Protein Array: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin beta 3 Antibody (SPTBN2/1584).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1584-Azide-and-BSA-Free-Protein-Array-NBP2-54439-img0009.jpg "Protein Array: Spectrin beta 3 Antibody (SPTBN2/1584) - Azide and BSA Free [NBP2-54439] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin beta 3 Antibody (SPTBN2/1584).")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")