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ADTB1 Antibody

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Western Blot: ADTB1 Antibody [NBP2-57109] - Analysis using Anti-AP1B1 antibody NBP2-57109 (A) shows similar pattern to independent antibody NBP2-57886 (B).
Independent Antibodies: Western Blot: ADTB1 Antibody [NBP2-57109] - Analysis using Anti-AP1B1 antibody NBP2-57109 (A) shows similar pattern to independent antibody NBP2-57886 (B).
Immunocytochemistry/ Immunofluorescence: ADTB1 Antibody [NBP2-57109] - Staining of human cell line HEK 293 shows localization to cytosol & the Golgi apparatus.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

        

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ADTB1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PPSAFVEGGRGVVHKSLPPRTASSESAESPETAPTGAPP
Predicted Species
Mouse (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AP1B1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ADTB1 Recombinant Protein Antigen (NBP2-57109PEP)

Reactivity Notes

Rat 87%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for ADTB1 Antibody

  • Adapter-Related Protein Complex 1 Subunit Beta-1
  • Adaptor Protein Complex AP-1 Subunit Beta-1
  • Adaptor-Related Protein Complex 1, Beta 1 Subunit
  • AP-1 Complex Subunit Beta-1
  • AP105A
  • AP1B1
  • BAM22
  • beta-1-adaptin
  • beta1-adaptin
  • Beta-Adaptin 1
  • beta-prime-adaptin
  • CLAPB2
  • Clathrin Assembly Protein Complex 1 Beta Large Chain
  • Golgi Adaptor HA1/AP1 Adaptin Beta Subunit
  • Plasma Membrane Adaptor HA2/AP2 Adaptor Beta Subunit

Background

Membrane vesicle formation is a process required for the endocytosis and biosynthesis of various secreted and membrane bound proteins. Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AP1B1