EMMPRIN/CD147 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 44-66 kDa, variably glycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily (1-4). Human EMMPRIN is 269 amino acids (aa) in length and contains a 24 aa signal sequence, a 183 aa extracellular domain (ECD), a 21 aa transmembrane (TM) domain and a 41 aa intracellular domain. The ECD contains one C2-type and one V-type Ig-like domain. There is one 385 aa splice variant that contains an extra N-terminal IgCAM domain and is found only in the retina (5). mRNA transcripts, but not protein, have been reported for additional 432, 388, 205, 176, and 174 aa variants.

EMMPRIN is expressed in areas of tissue remodeling, including tumors, endometrium, placenta, skin, and regions undergoing angiogenesis (1, 2, 6-9). It is also expressed in cells with high metabolic activity, such as lymphoblasts, macrophages and tumor cells (2, 10). On cells with elevated metabolic rates, EMMPRIN is often co-expressed with the amino acid transporter CD98h (11). EMMPRIN also interacts with caveolin-1 (via its C2-like domain), and this reduces the level of EMMPRIN glycosylation and subsequent EMMPRIN multimerization and activity (12). EMMPRIN’s TM sequence contains a Glu and a Pro which are important for intracellular interactions with cyclophilins (CyP) (3, 13, 14). CyPA (cyclosporin A receptor) and CyP60 interactions with the TM segment promote leukocyte inflammatory chemotaxis and surface expression of EMMPRIN, respectively (13, 14). An active 22 kDa fragment can be shed from tumor cells by MT1-MMP (1). Tumor cells can also release active, full-length EMMPRIN in microvesicles (15, 16). Functionally, EMMPRIN is known to induce urokinase-type plasminogen activator (uPA), VEGF, hyaluronan, and multiple MMPs (1, 2, 7, 8, 9). Human EMMPRIN (269 aa) shows 58%, 58%, 62%, and 52% aa identity with mouse, rat, bovine, and chicken EMMPRIN, respectively. It also shows 25% and 38% aa identity with the related proteins, embigin and neuroplastin (SDR-1), respectively (4).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ELISA Capture, ELISA Development, IHC, IHC-P, Immunoprecipitation, Western Blot.

Showing Publications 1 - 10 of 15. Show All 15 Publications. Collapse Publications.

Publications using AF972	Applications	Species
April Rees, Steve Turner, Catherine A. Thornton Potential protective effects of breast milk and amniotic fluid against novel coronavirus SARS‐CoV‐2 through decoy receptors Pediatric Allergy and Immunology 2022-01-01 [PMID: 34585801]
Elisabeth Flie beta er, Anna Birnhuber, Leigh M Marsh, Elisabeth Gschwandtner, Walter Klepetko, Horst Olschewski et al. Dysbalance of ACE2 levels – a possible cause for severe COVID‐19 outcome in COPD The Journal of Pathology: Clinical Research 2021-09-01 [PMID: 33978304]
N Sun, YT Lee, RY Zhang, R Kao, PC Teng, Y Yang, P Yang, JJ Wang, M Smalley, PJ Chen, M Kim, SJ Chou, L Bao, J Wang, X Zhang, D Qi, J Palomique, N Nissen, SB Han, S Sadeghi, RS Finn, S Saab, RW Busuttil, D Markovic, D Elashoff, HH Yu, H Li, AP Heaney, E Posadas, S You, JD Yang, R Pei, VG Agopian, HR Tseng, Y Zhu Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoring Nat Commun, 2020-09-07;11(1):4489. 2020-09-07 [PMID: 32895384] (ELISA Capture, Human)	ELISA Capture	Human
M Aoki, K Koga, M Miyazaki, M Hamasaki, N Koshikawa, M Oyama, H Kozuka-Hat, M Seiki, BP Toole, K Nabeshima CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts BMC Cancer, 2019-09-12;19(1):912. 2019-09-12 [PMID: 31510956] (Immunoprecipitation, Human)	Immunoprecipitation	Human
A Sugyo, AB Tsuji, H Sudo, M Koizumi, Y Ukai, G Kurosawa, Y Kurosawa, T Saga, T Higashi Efficacy Evaluation of Combination Treatment Using Gemcitabine and Radioimmunotherapy with 90Y-Labeled Fully Human Anti-CD147 Monoclonal Antibody 059-053 in a BxPC-3 Xenograft Mouse Model of Refractory Pancreatic Cancer Int J Mol Sci, 2018-09-29;19(10):. 2018-09-29 [PMID: 30274301] (IHC, Human)	IHC	Human
D Gong, X Chi, K Ren, G Huang, G Zhou, N Yan, J Lei, Q Zhou Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin Nat Commun, 2018-09-06;9(1):3623. 2018-09-06 [PMID: 30190470] (Western Blot, Human)	Western Blot	Human
Mirel-Adrian Popa, Maria-Cristina Mihai, Alina Constantin, Viorel Şuică, Cătălin Ţucureanu, Raluca Costache et al. Dihydrotestosterone induces pro-angiogenic factors and assists homing of MSC into the cardiac tissue Journal of Molecular Endocrinology 2018-01-01 [PMID: 29247133]
Wenjing Xiao, Shufen Zhao, Fangzhen Shen, Jun Liang, Jing Chen Overexpression of CD147 is associated with poor prognosis, tumor cell migration and ERK signaling pathway activation in hepatocellular carcinoma Experimental and Therapeutic Medicine 2017-09-01 [PMID: 28962206]
Cryopreserved Mesenchymal Stromal Cells Maintain Potency in a Retinal Ischemia/Reperfusion Injury Model: Toward an off-the-shelf Therapy Sci Rep, 2016-05-23;6(0):26463. 2016-05-23 [PMID: 27212469] (IHC-P, Mouse)	IHC-P	Mouse
Yau-Hua Yu, Jose Morales, Lei Feng, J. Jack Lee, Adel K. El-Naggar, Nadarajah Vigneswaran CD147 and Ki-67 overexpression confers poor prognosis in squamous cell carcinoma of oral tongue: A tissue microarray study Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 2015-05-01 [PMID: 25747176]
Aya Sugyo, Atsushi B. Tsuji, Hitomi Sudo, Kotaro Nagatsu, Mitsuru Koizumi, Yoshinori Ukai et al. Evaluation of (89)Zr-labeled human anti-CD147 monoclonal antibody as a positron emission tomography probe in a mouse model of pancreatic cancer PLoS ONE 2013-04-05 [PMID: 23577210]
Venkatesan B, Valente AJ, Reddy VS, Siwik DA, Chandrasekar B Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migration. Am. J. Physiol. Heart Circ. Physiol., 2009-06-26;297(2):H874-86. 2009-06-26 [PMID: 19561311] (ELISA Development, Human)	ELISA Development	Human
Koga K, Nabeshima K, Aoki M, Kawakami T, Hamasaki M, Toole BP, Nakayama J, Iwasaki H Emmprin in epithelioid sarcoma: expression in tumor cell membrane and stimulation of MMP-2 production in tumor-associated fibroblasts. Int. J. Cancer, 2007-02-15;120(4):761-8. 2007-02-15 [PMID: 17131322] (IHC-P, Human)	IHC-P	Human
Nabeshima K, Iwasaki H, Nishio J, Koga K, Shishime M, Kikuchi M Expression of emmprin and matrix metalloproteinases (MMPs) in peripheral nerve sheath tumors: emmprin and membrane-type (MT)1-MMP expressions are associated with malignant potential. Anticancer Res., 2006-03-01;26(2):1359-67. 2006-03-01 [PMID: 16619545] (IHC-P, Human)	IHC-P	Human
Pushkarsky T, Zybarth G, Dubrovsky L, Yurchenko V, Tang H, Guo H, Toole B, Sherry B, Bukrinsky M CD147 facilitates HIV-1 infection by interacting with virus-associated cyclophilin A. Proc. Natl. Acad. Sci. U.S.A., 2001-05-15;98(11):6360-5. 2001-05-15 [PMID: 11353871] (Immunoprecipitation, Human)	Immunoprecipitation	Human
Show All 15 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	IHC-Fr	Mouse	01/12/2015	View Summary Application Immunohistochemistry-Frozen Sample Tested See PMID 24527394 Species Mouse

Array AF972

• EMMPRIN/CD147 Antibodies
• EMMPRIN/CD147 Antibody Pair
• EMMPRIN/CD147 Lysates
• EMMPRIN/CD147 Proteins
• EMMPRIN/CD147 ELISAs
• EMMPRIN/CD147 Proteins and Enzymes