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Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric)

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Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric) [NBP2-77004] - Standard Curve Reference

Product Details

Summary
Reactivity ShSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum and plasma
Standard Curve Range
6.25 ng/mL - 100 ng/mL
Sensitivity
3.125 ng/mL

Order Details

Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of Sheep Adiponectin/Acrp30. No significant cross-reactivity or interference between Sheep Adiponectin/Acrp30 and analogues was observed.
Standard Curve Range
6.25 ng/mL - 100 ng/mL
Sensitivity
3.125 ng/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Spike Recovery
84 - 103%
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ADIPOQ

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay Plate
  3. HRP-Conjugate (100 x concentrate)
  4. HRP-Conjugate Diluent
  5. Instruction Manual
  6. Sample Diluent
  7. Standard (10 x concentrate)
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (25 x concentrate)

Alternate Names for Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric)

  • ACDC
  • Acrp30
  • ACRP30ADPN
  • adipocyte, C1Q and collagen domain containing
  • Adiponectin
  • adiponectin, C1Q and collagen domain containing
  • AdipoQ
  • ADIPQTL1
  • ApM1
  • apM-1
  • APM1APM-1
  • C1q and collagen domain-containing protein
  • GBP28
  • GBP28apM1
  • Gelatin-binding protein

Background

Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ADIPOQ