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Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free

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Western Blot: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adiponectin (Right lane) cDNA for 48 hrs and ...read more
Immunocytochemistry/ Immunofluorescence: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Adiponectin.
Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Human Kidney tissue using anti-Adiponectin mouse monoclonal antibody.
Flow Cytometry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by ...read more
Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Adiponectin mouse monoclonal antibody.
Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Adiponectin mouse monoclonal antibody.
Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Adiponectin mouse monoclonal antibody.
Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Human breast tissue using anti-Adiponectin mouse monoclonal antibody.
Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Human liver tissue using anti-Adiponectin mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1H1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 19-244 of human ADIPOQ(NP_004788) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ADIPOQ
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
24.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free

  • ACDC
  • Acrp30
  • ACRP30ADPN
  • adipocyte, C1Q and collagen domain containing
  • Adiponectin
  • adiponectin, C1Q and collagen domain containing
  • AdipoQ
  • ADIPQTL1
  • ApM1
  • apM-1
  • APM1APM-1
  • C1q and collagen domain-containing protein
  • GBP28
  • GBP28apM1
  • Gelatin-binding protein

Background

Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ADIPOQ