![Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Western-Blot-NBP1-90361-img0005.jpg "Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: QVELHVHLDGSIKPETILYYGRRRGIALPANTAEGLLNVIGMDKPLTLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEGERDFGVKARSILCC |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ADA |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Application Notes | Use in ICC/IF reported in scientific literature (PMID:34445661). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF fixation permeabilization: PFA/Triton X-100. |
||
| Control Peptide |
|
||
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
| Publication using NBP1-90361 | Applications | Species |
|---|---|---|
| Sohn R, Junker M, Meurer A Et al. Anti-Inflammatory Effects of Endogenously Released Adenosine in Synovial Cells of Osteoarthritis and Rheumatoid Arthritis Patients International journal of molecular sciences 2021-08-19 [PMID: 34445661] (ICC/IF, Human) | ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Adenosine Deaminase/ADA Antibody (NBP1-90361)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ADA |
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Analysis in human duodenum and testis tissues using NBP1-90361 antibody. Corresponding Adenosine Deaminase/ADA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-90361-img0017.jpg "Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Analysis in human duodenum and testis tissues using NBP1-90361 antibody. Corresponding Adenosine Deaminase/ADA RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human duodenum shows moderate to strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-90361-img0007.jpg "Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human duodenum shows moderate to strong cytoplasmic and nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-90361-img0008.jpg "Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-90361-img0009.jpg "Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human colon shows no positivity in glandular cells as expected.")
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-90361-img0010.jpg "Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.")
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-90361-img0012.jpg "Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human lymph node.")
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human colon using Anti-ADA antibody NBP1-90361.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-90361-img0013.jpg "Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human colon using Anti-ADA antibody NBP1-90361.")
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human duodenum using Anti-ADA antibody NBP1-90361.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-90361-img0014.jpg "Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human duodenum using Anti-ADA antibody NBP1-90361.")
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human testis using Anti-ADA antibody NBP1-90361.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-90361-img0015.jpg "Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-90361] - Staining of human testis using Anti-ADA antibody NBP1-90361.")
![Simple Western DPPIV/CD26 Antibody [Unconjugated]](https://images.novusbio.com/images2/DPPIV_AF1180_Simple_Western_20720.jpg)
![Immunohistochemistry DPPIV/CD26 Antibody [Unconjugated]](https://images.novusbio.com/images2/DPPIV_AF1180_Immunohistochemistry_17209.jpg)
![Immunocytochemistry DPPIV/CD26 Antibody [Unconjugated]](https://images.novusbio.com/images2/DPPIV_AF1180_Immunocytochemistry__Immunofluorescence_17210.jpg)
![Western Blot Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_Western_Blot_10680.jpg)
![Immunohistochemistry Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_Immunohistochemistry_10732.jpg)
![ELISA Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_ELISA_22233.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")