Home » Acetyl CoA synthetase » Acetyl CoA synthetase Lysates » Acetyl CoA synthetase Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

Acetyl CoA synthetase Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: Acetyl CoA synthetase Overexpression Lysate (Adult Normal) [NBL1-07270] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Acetyl CoA synthetase.
Western Blot: Acetyl CoA synthetase Overexpression Lysate (Adult Normal) [NBL1-07270] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Acetyl CoA synthetase.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Acetyl CoA synthetase Overexpression Lysate Summary

Description

Acetyl CoA synthetase Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC204260

Accession#: NM_018677

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
ACSS2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (cat# NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
78.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for Acetyl CoA synthetase Overexpression Lysate

  • ACAS2
  • AceCS
  • acetate thiokinase
  • acetate-CoA ligase
  • Acetate--CoA ligase
  • Acetyl-CoA synthetase
  • acetyl-Coenzyme A synthetase 2 (ADP forming)
  • acetyl-coenzyme A synthetase, cytoplasmic
  • ACSA
  • ACSACECS
  • Acyl-activating enzyme
  • acyl-CoA synthetase short-chain family member 2EC 6.2.1.1
  • cytoplasmic acetyl-coenzyme A synthetase
  • dJ1161H23.1
  • DKFZp762G026
  • EC 6.2.1

Background

This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the predominant isoform (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

C-Reactive
Protein/CRP [Biotin]
DY1707
N/A C-Reactive Protein/CRP [Biotin]
(68 Publications)
Species: Hu
Applications: ELISA
Serpin A5/Protein
C Inhibitor Antibody ...
MAB1266
Intracellular Staining by Flow Cytometry Serpin A5/Protein C Inhibitor Antibody (210326) [Unconjugated]
(2 Publications)
Species: Hu
Applications: CyTOF-ready, IP, ICFlow, WB
CSRP1 Antibody
AF5739
Western Blot CSRP1 Antibody
Species: Hu, Mu, Rt
Applications: WB
BMP-2
[Unconjugated]
355-BM
Bioactivity BMP-2 [Unconjugated]
(189 Publications)
Species: Hu, Mu, Rt
Applications: BA
MITF Antibody
AF5769
Western Blot MITF Antibody
Immunohistochemistry MITF Antibody
(9 Publications)
Species: Hu
Applications: IHC, WB
ACE/CD143 Antibody
[Unconjugated]
AF1513
Immunohistochemistry ACE/CD143 Antibody [Unconjugated]
Western Blot ACE/CD143 Antibody [Unconjugated]
Simple Western ACE/CD143 Antibody [Unconjugated]
(1 Review)
(7 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(645 Publications)
Species: Mu
Applications: ELISA
ACSL5 Antibody
(5H8)
H00051703-M01
Western Blot ACSL5 Antibody (5H8)
Western Blot ACSL5 Antibody (5H8)
Immunohistochemistry-Paraffin ACSL5 Antibody (5H8)
(6 Publications)
Species: Hu
Applications: ELISA, IHC, IHC-P, IP, S-ELISA, WB
Kallikrein 3/PSA
[HRP]
DKK300
N/A Kallikrein 3/PSA [HRP]
N/A Kallikrein 3/PSA [HRP]
N/A Kallikrein 3/PSA [HRP]
(17 Publications)
Species: Hu
Applications: ELISA
Acetyl-CoA
Carboxylase alpha/ACA ...
MAB6898
Western Blot Acetyl-CoA Carboxylase alpha/ACACA Antibody (738421)
(3 Publications)
Species: Hu, Mu
Applications: WB
ACSL1 Antibody
NBP1-89270
Immunocytochemistry/ Immunofluorescence ACSL1 Antibody
Immunohistochemistry-Paraffin ACSL1 Antibody
Western Blot ACSL1 Antibody
(1 Review)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(809 Publications)
Species: Hu
Applications: BA
CD40 Ligand/TNFSF5
[HRP]
DCDL40
N/A CD40 Ligand/TNFSF5 [HRP]
N/A CD40 Ligand/TNFSF5 [HRP]
(44 Publications)
Species: Hu
Applications: ELISA
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(24 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
ACSL6 Antibody
NBP2-15253
Western Blot ACSL6 Antibody
(1 Review)
Species: Mu, Rt
Applications: WB

Myeloperoxidase/MPO An ...
AF3667
Western Blot Myeloperoxidase/MPO Antibody [Unconjugated]
Simple Western Myeloperoxidase/MPO Antibody [Unconjugated]
Immunocytochemistry Myeloperoxidase/MPO Antibody [Unconjugated]
(3 Reviews)
(165 Publications)
Species: Hu, Mu
Applications: Dual ISH-IHC, ICC, IHC, Simple Western, WB
Coagulation Factor
III/Tissue Factor [HR ...
DCF300
N/A Coagulation Factor III/Tissue Factor [HRP]
N/A Coagulation Factor III/Tissue Factor [HRP]
(26 Publications)
Species: Hu
Applications: ELISA
Acetyl CoA
synthetase Overexpres ...
NBL1-07270
Acetyl CoA synthetase Overexpression Lysate
Species: Hu
Applications: WB

Publications for Acetyl CoA synthetase Lysate (NBL1-07270) (0)

There are no publications for Acetyl CoA synthetase Lysate (NBL1-07270).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Acetyl CoA synthetase Lysate (NBL1-07270) (0)

There are no reviews for Acetyl CoA synthetase Lysate (NBL1-07270). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Acetyl CoA synthetase Lysate (NBL1-07270) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Acetyl CoA synthetase Products

Research Areas for Acetyl CoA synthetase Lysate (NBL1-07270)

Find related products by research area.

Blogs on Acetyl CoA synthetase

There are no specific blogs for Acetyl CoA synthetase, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Acetyl CoA synthetase Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol ACSS2