![Western Blot: 5-HT4 Antibody [NBP1-78403] - Western Blot Image of anti-5HT4 Receptor. Total protein from human liver tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LYVE1 in 1% milk/TBST. Reactivity was detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/5-HT4-Antibody-Western-Blot-NBP1-78403-img0003.jpg "Western Blot: 5-HT4 Antibody [NBP1-78403] - Western Blot Image of anti-5HT4 Receptor. Total protein from human liver tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LYVE1 in 1% milk/TBST. Reactivity was detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.09 mg/ml |
| Immunogen | A synthetic peptide made to a C-terminal portion of the human 5HT4 Receptor protein (between residues 350-388) [UniProt Q13639, isoform 1] |
| Localization | Cell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HTR4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This 5HT4 Receptor antibody is useful for Immunocytochemistry/Immunofluorescence where cytoplasmic staining is observed in HeLa cells. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS and 30% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.09 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-78403 | Applications | Species |
|---|---|---|
| Omote M, Wakimoto Y, Shibahara H Possible Role of 5-Hydroxytryptamine (5-HT) Receptor on Human Sperm Motility Regulation Cureus 2023-11-01 [PMID: 38033435] (IHC) | IHC |
Secondary Antibodies |
Isotype Controls |
Research Areas for 5-HT4 Antibody (NBP1-78403)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: 5-HT4 Antibody [NBP1-78403] - Staining of 5HT4 receptor in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/5-HT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78403-img0004.jpg "Immunocytochemistry/Immunofluorescence: 5-HT4 Antibody [NBP1-78403] - Staining of 5HT4 receptor in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")