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11 beta-HSD1 Antibody

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Western blot shows lysates of human, mouse and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat 11 beta -HSD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3397) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for 11 beta -HSD1 at approximately 36 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Western blot shows lysates of human, mouse and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat 11 beta -HSD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3397) followed by ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF3397-SP
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11 beta-HSD1 Antibody Summary

Immunogen
E. coli-derived recombinant human 11 beta -HSD1
Asn24-Lys292
Accession # P28845
Specificity
Detects endogenous human, mouse and rat 11 beta -HSD1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HSD11B1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF3397 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 11 beta-HSD1 Antibody

  • 11 betaHSD1
  • 11 beta-HSD1
  • 11-beta-hydroxysteroid dehydrogenase 1
  • EC 1.1.1
  • EC 1.1.1.146
  • HDL
  • HSD11B1
  • HSD11HSD11B
  • HSD11LMGC13539
  • hydroxysteroid (11-beta) dehydrogenase 1
  • member 1
  • SDR26C1

Background

Hydoxysteroid (11-beta) Dehydrogenase, type I 11-beta-hydroxysteroid dehydrogenase 1(11 beta -HSD1, HSD11B1 or HSD1) is a microsomal glycoprotein enzyme, with an apparent mass of 36 kDa, that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. 11 beta -HSD1 can also catalyze the reverse reaction, the conversion of cortisone to cortisol. 11 beta -HSD1 has been detected in a wide range of rat and human tissues, including lung and testis, but highest amounts are expressed in the liver. Human 11 beta -HSD1 shares 79 and 77% sequence identity with mouse and rat 11 beta -HSD1, respectively. There are at least 2 isoforms of 11 beta -HSD. The other isoform, type II, is expressed predominantly in the kidney and placenta and catalyzes only the 11-beta-dehydrogenation reaction. High levels of cortisol can lead to visceral obesity and particular defects in the expression or function of 11 beta -HSD1 have also been associated with insulin resistance and cognitive function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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11 beta-HSD1
Antibody
AF3397
11 beta-HSD1 Antibody
Species: Hu, Mu, Rt
Applications: WB

Publications for 11 beta-HSD1 Antibody (AF3397)(6)

We have publications tested in 3 confirmed species: Human, Rat, Rabbit.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


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Immunoprecipitation
(1)
Western Blot
(3)
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(1)
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(2)
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(1)
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Showing Publications 1 - 6 of 6.
Publications using AF3397 Applications Species
Mengliang Hu, Tingting Han, Qiyu Pan, Dongsheng Ni, Fengyi Gao, Liying Wang et al. The GR-gp78 Pathway is involved in Hepatic Lipid Accumulation Induced by Overexpression of 11 beta -HSD1 International Journal of Biological Sciences 4/30/2022 [PMID: 35637957]
V Tesic, J Ciric, I Jovanovic, N Zogovic, D Milanovic, S Kanazir, M Perovic Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction Nutrients, 2021-12-17;13(12):. 2021-12-17 [PMID: 34960078] (Western Blot, Rat) Western Blot Rat
HC Huang, MH Tsai, FY Lee, TY Lin, CC Chang, CL Chuang, SJ Hsu, MC Hou, YH Huang NAFLD Aggravates Septic Shock Due to Inadequate Adrenal Response and 11beta-HSDs Dysregulation in Rats Pharmaceutics, 2020-04-28;12(5):. 2020-04-28 [PMID: 32354071] (Western Blot, Rat) Western Blot Rat
Rihua Zhang, Jing Bao Kan, Shan Lu, Pei Tong, Jie Yang, Ling Xi et al. S100A16-induced adipogenesis is associated with up-regulation of 11 beta -hydroxysteroid dehydrogenase type 1 (11 beta -HSD1) Bioscience Reports 9/30/2019 [PMID: 31399502]
Petrus P, Rosqvist F, Edholm D, Mejhert N, Arner P, Dahlman I, Ryden M, Sundbom M, Riserus U Saturated fatty acids in human visceral adipose tissue are associated with increased 11- beta-hydroxysteroid-dehydrogenase type 1 expression. Lipids Health Dis, 2015-05-02;14(0):42. 2015-05-02 [PMID: 25934644] (Western Blot, Human) Western Blot Human
Li B, Yau P, Kemper B Identification of cytochrome P450 2C2 protein complexes in mouse liver. Proteomics, 2011-08-01;11(16):3359-68. 2011-08-01 [PMID: 21751364] (Immunoprecipitation, Rabbit) Immunoprecipitation Rabbit

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Bioinformatics

Gene Symbol HSD11B1
Uniprot