Choose from nearly 1,000 secondary antibodies conjugated to more than 40 different enzymatic, fluorescent, or biotin labels. Use the filters on the left to sort our secondary antibodies by species, application, host, or conjugate.
To learn more about secondary antibodies, download and read our Secondary Antibody Handbook for technical information and tips to guide you when choosing a secondary antibody for your experiment.
To understand more about direct vs. indirect detection, including benefits of a secondary antibody and when to use one, please read our resources on Direct vs. Indirect Detection Methods.
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB7157 | |||
| Allophycocyanin | NB7156APC | |||
| Biotin | NB7158 | |||
| DyLight 350 | NB7156UV | |||
| DyLight 405 | NB7156V | |||
| DyLight 488 | NB7156G | |||
| DyLight 550 | NB7156R | |||
| DyLight 594 | NB7156DL594 | |||
| DyLight 650 | NB7156C | |||
| DyLight 680 | NB7156FR | |||
| DyLight 755 | NB7156IR | |||
| FITC | NB7159 | |||
| PE | NB7156PE | |||
| PerCP | NB7156PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB7157 | |||
| Allophycocyanin | NB7156APC | |||
| Biotin | NB7158 | |||
| DyLight 350 | NB7156UV | |||
| DyLight 405 | NB7156V | |||
| DyLight 488 | NB7156G | |||
| DyLight 550 | NB7156R | |||
| DyLight 594 | NB7156DL594 | |||
| DyLight 650 | NB7156C | |||
| DyLight 680 | NB7156FR | |||
| DyLight 755 | NB7156IR | |||
| FITC | NB7159 | |||
| HRP | NB7160 | |||
| PE | NB7156PE | |||
| PerCP | NB7156PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP Polymer | VC002-050 | |||
| HRP Polymer | VC002-125 | |||
| HRP Polymer | VC002-250 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | VC002-050 | |||
| Carrier Free | VC002-125 | |||
| Carrier Free | VC002-250 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-75294 | |||
| Biotin | NBP1-75295 | |||
| DyLight 350 | NBP1-76054 | |||
| DyLight 488 | NBP1-75299 | |||
| DyLight 550 | NBP1-76055 | |||
| DyLight 594 | NBP1-76056 | |||
| DyLight 633 | NBP1-76057 | |||
| DyLight 650 | NBP1-76058 | |||
| FITC | NBP1-75296 | |||
| HRP | NBP1-75297 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | D-301-C-ABS2 | |||
| Carrier Free | NL004 | |||
| Carrier Free | NL005 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-75281 | |||
| DyLight 350 | NBP1-75635 | |||
| DyLight 488 | NBP1-75285 | |||
| DyLight 550 | NBP1-75636 | |||
| DyLight 594 | NBP1-75637 | |||
| DyLight 633 | NBP1-75638 | |||
| FITC | NBP1-75282 | |||
| HRP | NBP1-75283 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-75281 | |||
| Pre-adsorbed | NBP1-75282 | |||
| Pre-adsorbed | NBP1-75283 | |||
| Pre-adsorbed | NBP1-75285 | |||
| Pre-adsorbed | NBP1-75635 | |||
| Pre-adsorbed | NBP1-75636 | |||
| Pre-adsorbed | NBP1-75637 | |||
| Pre-adsorbed | NBP1-75638 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | F0111 | |||
| Biotin | BAF008 | |||
| Fluorescein | F0112 | |||
| HRP | HAF008 | |||
| HRP Polymer | VC003-025 | |||
| HRP Polymer | VC003-050 | |||
| HRP Polymer | VC003-125 | |||
| HRP Polymer | VC003-250 | |||
| Phycoerythrin | F0110 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | F0110 | |||
| Carrier Free | F0111 | |||
| Carrier Free | F0112 | |||
| Carrier Free | HAF008 | |||
| Carrier Free | VC003-025 | |||
| Carrier Free | VC003-050 | |||
| Carrier Free | VC003-125 | |||
| Carrier Free | VC003-250 | |||
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NB7156] - Both samples are cultured mouse microglial cells. The red is stained cells while the grey are cells with no primary antibody added (but the secondary antibody is present). Flow cytometry image submitted by a verified customer review.. Using the FITC format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Flow-Cytometry-NB7156-img0001.jpg "Flow Cytometry: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NB7156] - Both samples are cultured mouse microglial cells. The red is stained cells while the grey are cells with no primary antibody added (but the secondary antibody is present). Flow cytometry image submitted by a verified customer review.. Using the FITC format of this antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Image from the Alkaline Phosphatase version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72732-img0021.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Image from the Alkaline Phosphatase version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - DyLight 488 conjugated anti-Rabbit IgG was used to demonstrate 2 color STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 ug/mL final concentration of anti-HDAC and detected with 0.2 ug/mL DyLight488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody (colored GREEN). Also shown in this 2-color STED image is a-tubulin monoclonal antibody [MOUSE] detected with ATTO 425 conjugated anti-MOUSE IgG [GOAT] secondary antibody (colored RED). Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72732-img0019.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG. Load: 100 ng per lane. Primary antibody: RABBIT IgG (H&L) Antibody Pre-adsorbed at 1:1,000 for overnight at 4C. Secondary antibody: DyLight 800 goat secondary antibody 1:20,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 and 28 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72732-img0007.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB7575] - Lane 1: Rabbit IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72732-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72732-img0018.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP1-72732-img0026.jpg)
![SDS-Page: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - SDS-Page of Goat anti-Rabbit IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed).Lane 1: Goat anti-Rabbit IgG - Non-Reduced.Lane 2: Goat anti-Rabbit IgG - Reduced.Load: 1.0 ug per lane.Predicted/Observed size: 25 & 55 kDa - Reduced, 160 kDa - Non-Reduced for Goat IgG.Other Band(s): None.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-SDS-Page-NBP1-72732-img0025.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Lysates of Jurkat human acute T cell leukemia cell line either untreated (-) or treated (+) with EPE. PVDF membrane was probed with 0.25 ug/mL rabbit anti-phospho RAD17 (S635) monoclonal antibody (MAB1374) followed by 1:1000 dilution of goat anti-rabbit IgG-HRP secondary antibody (NBP1-75297).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-75293-img0007.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Lysates of Jurkat human acute T cell leukemia cell line either untreated (-) or treated (+) with EPE. PVDF membrane was probed with 0.25 ug/mL rabbit anti-phospho RAD17 (S635) monoclonal antibody (MAB1374) followed by 1:1000 dilution of goat anti-rabbit IgG-HRP secondary antibody (NBP1-75297).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Lysates of Jurkat human acute T cell leukemia cell line either untreated (-) or treated (+) with EPE. PVDF membrane was probed with 0.25 ug/mL rabbit anti-phospho RAD17 (S635) monoclonal antibody (MAB1374) followed by 1:1000 dilution of Goat anti-Rabbit IgG (H+L) Secondary Antibody (NBP1-75297).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-75293-img0005.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Analysis of WNV-E in A549 cell lysate using Goat anti-Rabbit IgG (H+L) Secondary Antibody. The secondary antibody was diluted in 5% milk at a dilution of 1:3000. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-75293-img0004.jpg)
![Western Blot: Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72763] - Western Blot of Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed) 1:500 for 60 min at RT. Secondary antibody: Anti-Guinea Porcine IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab). Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Guinea-Pig-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72763-img0009.jpg "Western Blot: Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72763] - Western Blot of Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed) 1:500 for 60 min at RT. Secondary antibody: Anti-Guinea Porcine IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab). Rabbit F(c) migrates slightly higher.")
![ELISA: Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72763] - ELISA Results of Purified Guinea Porcine Anti-Rabbit IgG mx3 Antibody tested against purified Rabbit IgG (Mx Human IgG, Goat IgG, Mouse IgG). Each well was coated in duplicate with 10 ug of Rabbit IgG [Red Line], Human IgG [Green Line], Goat IgG [Blue Line], Mouse IgG [Purple Line]. The working dilution is 1:17,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Guinea Porcine mx10 HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Guinea-Pig-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72763-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - West Nile virus infection of mosquito cells were stained with primary antibody followed by DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75279-img0006.jpg "Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - West Nile virus infection of mosquito cells were stained with primary antibody followed by DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 form of this antibody.")
![Immunohistochemistry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of mouse tissue. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-NBP1-75279-img0008.jpg)
![Flow Cytometry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of human pancreatic cancer cells. Note: Need to be careful about bleeding into different filters since it might interfere with FITC. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Flow-Cytometry-NBP1-75279-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of human osteosarcoma cell line. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75279-img0005.jpg)
![Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of PP4C in Huh4 cell lysate. Image from verified customer review. Image using the HRP format of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-75286-img0009.jpg "Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of PP4C in Huh4 cell lysate. Image from verified customer review. Image using the HRP format of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of human osteosarcoma cell using Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75286-img0006.jpg)
![Immunohistochemistry-Frozen: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of frozen mouse tissue using DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-Frozen-NBP1-75286-img0008.jpg)
![Flow Cytometry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - analysis of LM7 cells using Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) conjugated to DyLight 594. Image from verified customer review. Image using the DyLight 594 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Flow-Cytometry-NBP1-75286-img0007.jpg)
![Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB120-6826] - Lane 1: Rabbit IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine chicken secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Chicken-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6831-img0002.jpg "Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB120-6826] - Lane 1: Rabbit IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine chicken secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa for Rabbit IgG.")
![Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Lane 1: Rabbit IgG. Load: 50 ug per lane. Secondary antibody: Texas Red (TM) chicken secondary antibody at 1:1,000 for 60 min at RT. Block for30 min at RT. Predicted/Observed size: 55 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Chicken-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6831-img0006.jpg)
![Western Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (CHICKEN) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Chicken IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Chicken-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6831-img0004.jpg)
![Dot Blot: Chicken anti-Rabbit IgG (H+L) Secondary Antibody [NB120-6831] - Dot Blot of Chicken anti-Rabbit IgG (H+L) Secondary Antibody [Fluorescein] (Pre-absorbed). Antigen: Rabbit IgG Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Incubated for 1hr at RT; blocked for 60 min at RT. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Chicken-anti-Rabbit-IgG-H+L-Secondary-Antibody-Dot-Blot-NB120-6831-img0005.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75321] - Total cell lysate from human UMUC1. This image was submitted via customer Review. Image from the HRP version of Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-75321-img0004.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75321] - Total cell lysate from human UMUC1. This image was submitted via customer Review. Image from the HRP version of Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).")