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Goat anti-Rabbit IgG Secondary Antibody [Unconjugated]

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Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Goat anti-Rabbit IgG Secondary Antibody [Unconjugated] Summary

Immunogen
Rabbit IgG
Specificity
Detects rabbit IgG heavy and light chains.  In direct ELISAs, less than 5% cross-reactivity with mouse IgG is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Western Blot 1:5000 dilution
Publications
Read Publications using
AF008 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Rabbit IgG Secondary Antibody [Unconjugated]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (AF008)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 5 applications: ChIP, Flow Cytometry, ICC, IHC, Western Blot.


Filter By Application
ChIP
(1)
Flow Cytometry
(1)
ICC
(1)
IHC
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF008 Applications Species
N Fakatava, H Mitarai, A Yuda, A Haraguchi, H Wada, D Hasegawa, H Maeda, N Wada Actin alpha 2, smooth muscle, a transforming growth factor-beta1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway Journal of dental sciences, 2023;18(2):567-576. 2023 [PMID: 37021273] (Western Blot, Human) Western Blot Human
N Fakatava, H Mitarai, A Yuda, A Haraguchi, H Wada, D Hasegawa, H Maeda, N Wada Actin alpha 2, smooth muscle, a transforming growth factor-beta1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway Journal of dental sciences, 2022-09-16;18(2):567-576. 2022-09-16 [PMID: 37021273] (Western Blot, Human) Western Blot Human
Mansky RH, Greguske EA, Yu D et al. Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease Cell reports 3/2/2023 [PMID: 36867535]
N Zarate, TA Intihar, D Yu, J Sawyer, W Tsai, M Syed, L Carlson, R Gomez-Past Heat Shock Factor 1 Directly Regulates Postsynaptic Scaffolding PSD-95 in Aging and Huntington's Disease and Influences Striatal Synaptic Density International Journal of Molecular Sciences, 2021-12-04;22(23):. 2021-12-04 [PMID: 34884918] (ChIP, Human, Mouse, Transgenic Mouse) ChIP Human, Mouse, Transgenic Mouse
S Wu, M Luo, KKW To, J Zhang, C Su, H Zhang, S An, F Wang, D Chen, L Fu Intercellular transfer of exosomal wild type EGFR triggers osimertinib resistance in non-small cell lung cancer Molecular Cancer, 2021-01-18;20(1):17. 2021-01-18 [PMID: 33461557] (ICC, Western Blot, Human) ICC, Western Blot Human
Tripathi A, Davis J, Staren D, Volkman B, Majetschak M CXC chemokine receptor 4 signaling upon co-activation with stromal cell-derived factor-1alpha and ubiquitin. Cytokine, 2013-12-24;65(2):121-5. 2013-12-24 [PMID: 24373940] (Flow Cytometry, Human) Flow Cytometry Human
Ruth JH, Haas CS, Park CC, Amin MA, Martinez RJ, Haines GK, Shahrara S, Campbell PL, Koch AE CXCL16-mediated cell recruitment to rheumatoid arthritis synovial tissue and murine lymph nodes is dependent upon the MAPK pathway. Arthritis Rheum., 2006-03-01;54(3):765-78. 2006-03-01 [PMID: 16508941] (IHC, Human) IHC Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IgG Antibody (AF008) (0)

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Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Bioinformatics

Gene Symbol IGHG1