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Goat anti-Rabbit IgG Secondary Antibody [HRP Polymer] (IHC-Prediluted)

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SKG was detected in paraffin-embedded sections of human kidney using Rabbit Anti-Human SGK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3200) at 3 μg/mL for 1 hour at room temperature followed byincubation for 30 minutes at room temperature with Anti-Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003). Tissue was stained with DAB (brown color) andcounterstained with hematoxylin (blue color).
SKG was detected in paraffin-embedded sections of human kidney using Rabbit Anti-Human SGK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3200) at 3 μg/mL for 1 hour at room temperature followed ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP Polymer
Format
IHC-Prediluted

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Allophycocyanin
F0111
Biotin
BAF008
Fluorescein
F0112
HRP
HAF008
HRP Polymer
VC003-050
HRP Polymer
VC003-125
HRP Polymer
VC003-250
Phycoerythrin
F0110
Unconjugated
AF008
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF008
Carrier Free
AF008
Carrier Free
F0110
Carrier Free
F0111
Carrier Free
F0112
Carrier Free
HAF008
Carrier Free
VC003-050
Carrier Free
VC003-125
Carrier Free
VC003-250
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Goat anti-Rabbit IgG Secondary Antibody [HRP Polymer] (IHC-Prediluted) Summary

Immunogen
Rabbit IgG
Specificity
Detects heavy ( gamma ) chains on rabbit IgG and light chains on all rabbit immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin rabbit serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Immunohistochemistry
Publications
Read Publications using
VC003 in the following applications:

  • IHC
    3 publications

Packaging, Storage & Formulations

Storage
6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied as a solution in PBS with preservative.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Rabbit IgG Secondary Antibody [HRP Polymer] (IHC-Prediluted)

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (VC003)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(3)
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Filter By Species
Human
(2)
Mouse
(1)
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