Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 7 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-FrFl, IHC-P, Immunocytochemistry/ Immunofluorescence, WB.

Showing Publications 1 - 10 of 22. Show All 22 Publications. Collapse Publications.

Publications using NBP1-72732	Applications	Species
Reddy PH, Kshirsagar S, Bose C et al. Rlip Reduction Induces Oxidative Stress and Mitochondrial Dysfunction in Mutant Tau-Expressed Immortalized Hippocampal Neurons: Mechanistic Insights Cells 2023-06-16 [PMID: 37371116] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Rueda-Gensini L, Serna JA, Rubio D et al. Three-dimensional neuroimmune co-culture system for modeling Parkinson's Disease microenvironments in vitro Biofabrication 2023-06-27 [PMID: 37369196] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Oliva Cárdenas A, Zamora-Rodríguez BC, Batalla-García KA et al. Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats Journal of visualized experiments : JoVE 2023-04-07 [PMID: 37092839]
Belvin BR, Lewis JP. Ferroportin depletes iron needed for cell cycle progression in head and neck squamous cell carcinoma Frontiers in Oncology 2023-01-09 [PMID: 36698390] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Yang Y, Chen C, Zuo Q Et al. NARF is a hypoxia-induced coactivator for OCT4-mediated breast cancer stem cell specification Sci Adv 2022-12-09 [PMID: 36490339] (ICC/IF)	ICC/IF
Atzori MG, Ceci C, Ruffini F Et al. The Anti-Vascular Endothelial Growth Factor Receptor 1 (VEGFR-1) D16F7 Monoclonal Antibody Inhibits Melanoma Adhesion to Soluble VEGFR-1 and Tissue Invasion in Response to Placenta Growth Factor Cancers (Basel) 2022-11-26 [PMID: 36428669] (IHC-P) Details: Citation using the HRP version of this antibody.	IHC-P
Zhang F, Zang TM, Stevenson EM et al. Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteins Proceedings of the National Academy of Sciences 2022-10-11 [PMID: 36136978]
Certel SJ, McCabe BD, Stowers RS. Et al. A conditional GABAergic synaptic vesicle marker for Drosophila J Neurosci Methods 2022-02-27 [PMID: 35219770] (IF/IHC) Details: Citation using the Janelia Fluor 549 version of this antibody.	IF/IHC
Certel SJ, Ruchti E, McCabe BD, Stowers RS. A conditional glutamatergic synaptic vesicle marker for Drosophila G3 Genes|Genomes|Genetics 2022-03-04 [PMID: 35100385]
Arguello T, Peralta S, Antonicka H et al. ATAD3A has a scaffolding role regulating mitochondria inner membrane structure and protein assembly Cell reports 2021-12-21 [PMID: 34936866]
Brady J, Vasin A, Bykhovskaia M. Et al. The Accessory Helix of Complexin Stabilizes a Partially Unzippered State of the SNARE Complex and Mediates the Complexin Clamping Function In Vivo eNeuro 2021-03-11 [PMID: 33692090] (IF/IHC) Details: Citation using the Cy3 version of this antibody.	IF/IHC
Wilson RH, Carney PR, Glover E, et al. Generation of an Allelic Series at the Ahr Locus Using an Edited Recombinant Approach Toxicol Sci 2021-01-22 [PMID: 33480436] (WB)	WB
Liu JT, Balmori-Cedeno J, Misk E, Lumsden JS Pharmacological and nutritional modulation of autophagy in a rainbow trout (Oncorhynchus mykiss) gill cell line, RTgill-W1 In Vitro Cell Dev Biol Anim 2020-09-09 [PMID: 32901427] (ICC/IF)	ICC/IF
Zhou S, Wang R, Xiao H Adipocytes induce the resistance of ovarian cancer to carboplatin through ANGPTL4 Oncol Rep 2020-06-16 [PMID: 32705217] (ICC/IF) Details: Citation using the Rhodamine format of this antibody.	ICC/IF
McKinney H. M, Sherer L. M, et al. Characterization of Drosophila octopamine receptor neuronal expression using MiMIC-converted Gal4 lines. J Comp Neurol 2020-09-01 [PMID: 32060912] Details: Citation using the Janiela Fluor 549 format of this antibody.
Tison K. V, McKinney H. M, et al. Demonstration of a Simple Epitope Tag Multimerization Strategy for Enhancing the Sensitivity of Protein Detection Using Drosophila vAChT. G3 (Bethesda) 2020-02-06 [PMID: 31767639] (ICC/IF)	ICC/IF
Posteraro B, Paroni Sterbini F, Petito V et al. Liver Injury, Endotoxemia, and Their Relationship to Intestinal Microbiota Composition in Alcohol-Preferring Rats Alcohol Clin Exp Res 2018-10-16 [PMID: 30320890] (ICC/IF)	ICC/IF
Zhu G, Wang J, Song M Et al. Overexpression of Jagged1 Ameliorates Aged Rat-Derived Endothelial Progenitor Cell Functions and Improves Its Transfusion Efficiency for Rat Balloon-Induced Arterial Injury Ann Vasc Surg 2017-02-07 [PMID: 28163178] Details: Citation using the Cy3 version of this antibody.
Garafola CS, Henn FA. A change in hippocampal protocadherin gamma expression in a learned helpless rat Brain Res 2014-12-03 [PMID: 25446008] (IHC-FrFl, IHC-Fr) Details: Citation using the Cy3 version of this antibody.	IHC-FrFl, IHC-Fr
Wang T, Gilkes DM, Takano N Et al. Hypoxia-inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis Proc Natl Acad Sci U S A 2014-06-19 [PMID: 24938788] (ICC/IF) Details: Citation using the Cy3 version of this antibody.	ICC/IF
Chen LL, Chen X, Choi H et al. Exploiting antitumor immunity to overcome relapse and improve remission duration. Cancer Immunol Immunother. [PMID: 22198309] Details: Citation using the Texas Red form of this antibody.
Rowland MM, Gong D, Bostic HE Et al. Microarray analysis of Akt PH domain binding employing synthetic biotinylated analogs of all seven phosphoinositide headgroup isomers Chem Phys Lipids 2011-12-20 [PMID: 22178158] Details: Citation using the Cy3 version of this antibody.
Show All 22 Publications. Collapse Publications.

Array NBP1-72732

• IgG (H+L) Antibodies