Goat anti-Rabbit IgG (H+L) Secondary Antibody

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 applications: FLOW, ICC/IF, WB.

Showing Publications 1 - 10 of 14. Show All 14 Publications. Collapse Publications.

Publications using NBP1-75293	Applications	Species
Bandopadhyay S, Kamal IM, Padmanaban E et al. Oncogene-mediated nuclear accumulation of lactate promotes epigenetic alterations to induce cancer cell proliferation Journal of cellular biochemistry 2023-04-01 [PMID: 36999756]
Kwon J, Yeh Y, Kawarasaki S et al. Mevalonate biosynthesis pathway regulates the development and survival of brown adipocytes iScience 2023-03-01 [PMID: 36895651] (WB)	WB
Xu W, Tang J, Zhao L DNA-protein cross-links between abasic DNA damage and mitochondrial transcription factor A (TFAM) Nucleic acids research 2022-12-30 [PMID: 36583367] (WB) Details: Dilution used in WB 1:1000	WB
Ng SP, Nomura W, Takahashi H Et al. Methylglyoxal induces multiple serine phosphorylation in insulin receptor substrate 1 via the TAK1-p38-mTORC1 signaling axis in adipocytes Biochem J 2022-10-18 [PMID: 36256829] Details: Citation using the HRP version of this antibody.
Madsen CS, Makela AV, Greeson EM Et al. Engineered endosymbionts that alter mammalian cell surface marker, cytokine and chemokine expression Commun Biol 2022-08-30 [PMID: 36042261] (ICC/IF) Details: Citation using the DyLight 650 version of this antibody.	ICC/IF
Yang W, Han B, Chen Y, Geng F SAAL1, a novel oncogene, is associated with prognosis and immunotherapy in multiple types of cancer Aging 2022-08-13 [PMID: 35963646] (WB)	WB
Ng SP, Nomura W, Takahashi H Et al. Methylglyoxal attenuates isoproterenol-induced increase in uncoupling protein 1 expression through activation of JNK signaling pathway in beige adipocytes Biochem Biophys Rep 2021-09-16 [PMID: 34527816] (WB) Details: Citation using the HRP version of this antibody.	WB
Gomaa AA, El-Abhar HS, Abdallah DM et al. Prasugrel anti-ischemic effect in rats: Modulation of hippocampal SUMO2/3-I kappa B alpha/Ubc9 and SIRT-1/miR-22 trajectories Toxicol Appl Pharmacol 2021-06-26 [PMID: 34174262] Details: This citation used the HRP format of this antibody.
Salem MA, Ismail RS, Zaki HF et al. L-carnitine extenuates endocrine disruption, inflammatory burst and oxidative stress in carbendazim-challenged male rats via upregulation of testicular StAR and FABP9, and downregulation of P38-MAPK pathways Toxicology 2021-05-09 [PMID: 33965443] Details: Citation using the HRP format of this antibody.
Khera E, Cilliers C, Smith MD et al Quantifying ADC bystander payload penetration with cellular resolution using pharmacodynamic mapping Neoplasia 2021-01-01 [PMID: 33385970] (FLOW)	FLOW
Amano H, Mastui Y, Ito Y et al. The role of vascular endothelial growth factor receptor 1 tyrosine kinase signaling in bleomycin-induced pulmonary fibrosis Biomed Pharmacother 2019-06-09 [PMID: 31176171] (WB)	WB
Khedr RM, Ahmed AAE, Kamel R et al. Sitagliptin attenuates intestinal ischemia/reperfusion injury via cAMP/PKA, PI3K/Akt pathway in a glucagon-like peptide 1 receptor-dependent manner Life Sci 2018-09-09 [PMID: 30195035] (WB)	WB
Luo B, Wang J, Liu Z et al. Phagocyte respiratory burst activates macrophage erythropoietin signalling to promote acute inflammation resolution. Nat Commun. [PMID: 27397585] (WB) Details: Citation using the HRP form of this antibody.	WB
Luo B, Jiang M, Yang X et al. Erythropoietin is a hypoxia inducible factor-induced protective molecule in experimental autoimmune neuritis Biochim Biophys Acta 2013-04-23 [PMID: 23603807] (WB)	WB
Show All 14 Publications. Collapse Publications.

Array NBP1-75293

• IgG (H+L) Antibodies