Donkey anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 488] (Pre-adsorbed)

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 applications: ICC/IF, IF/IHC, IHC-P.

Showing Publications 1 - 5 of 5.

Publications using NBP1-75292	Applications	Species
Bassiouni M, Smorodchenko A, Olze H, Szczepek AJ Identification and Characterization of TMEM119-Positive Cells in the Postnatal and Adult Murine Cochlea Brain sciences 2023-03-20 [PMID: 36979326] (ICC/IF)	ICC/IF
Makuch S, Kupczyk P, Makarec A et al. The Impact of Proinflammatory Cytokines and Imiquimod on GLUT1 in HaCaT Keratinocytes - a Potential Anti-Psoriatic Therapeutic Target? Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2023-03-22 [PMID: 36945896] (ICC/IF)	ICC/IF
Henning NF, LeDuc RD, Even KA et al. Proteomic analyses of decellularized porcine ovaries identified new matrisome proteins and spatial differences across and within ovarian compartments Sci Rep 2019-12-29 [PMID: 31882863] (IHC-P)	IHC-P
England CG, Jiang D, Ehlerding EB et al. (89)Zr-labeled nivolumab for imaging of T-cell infiltration in a humanized murine model of lung cancer Eur J Nucl Med Mol Imaging 2017-08-20 [PMID: 28821924] (IF/IHC)	IF/IHC
Henning N The Ovary: Physical and Biochemical Properties Controlling Folliculogenesis Thesis 2022-01-01

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: STEFANO Benvegnu	IHC-Fr	Mouse	06/29/2022	View Summary Application Immunohistochemistry-Frozen Sample Tested Adult mouse brain Species Mouse Lot 86-155-020722 Comments Comments Mouse brain tissue (12um thickness) was incubated overnight with anti-CD31 antibody (raised in rabbit) in PBS 1x at 4C to stain for brain endothelial cells. The day after, the slide was washed 3 x PBS 1x and then incubated with secondary antibody NBP1-75292, 1:1000 for 2 hours at r.t. After counterstaining with DAPI, image was acquired with confocal microscopy. Counterstained image with DAPI is shown.

Array NBP1-75292

• IgG (H+L) Antibodies