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Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed)

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Western Blot: Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72763] - Western Blot of Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane ...read more
ELISA: Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72763] - ELISA Results of Purified Guinea Porcine Anti-Rabbit IgG mx3 Antibody tested against purified Rabbit IgG (Mx Human IgG, Goat IgG, ...read more
Western Blot of Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed)

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Guinea Pig
Conjugate
Unconjugated
Format
Pre-adsorbed

Order Details

Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) Summary

Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum, Rabbit IgG and Rabbit Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Immunogen
Rabbit IgG whole molecule
Specificity
This antibody was pre-adsorbed against Human, Goat, and Mouse Serum Proteins.

No reaction was observed against Goat, Human and Mouse Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Guinea Pig
Purity
Multi-step

Applications/Dilutions

Dilutions
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:2000 - 1:10000
Application Notes
This secondary antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
Publications
Read Publications using
NBP1-72763 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Multi-step

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP1-72763)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: CHIP-SEQ, ChIP, Chip Cytometry.


Filter By Application
CHIP-SEQ
(1)
ChIP
(1)
Chip Cytometry
(2)
All Applications
Filter By Species
Human
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP1-72763 Applications Species
Zhang Y, Zang Y, Chen J et al. A Truncated ETHYLENE INSENSITIVE3/EIN3-Like protein, GhLYI, Regulates Senescence in Cotton Plant physiology 2023-07-11 [PMID: 37430389]
Zhang B, Srivastava A, Mimitou E et al. Characterizing cellular heterogeneity in chromatin state with scCUT&Tag-pro Nature Biotechnology 2022-08-01 [PMID: 35332340]
Courret C, Hemmer L, Wei X et al. Rapid turnover of centromeric DNA reveals signatures of genetic conflict in Drosophila bioRxiv 2023-08-23 (ChIP)

Details:
1:100 ChIP dilution
ChIP
Hunold P, Hoehne M, Kiljan M et al. G-quadruplex DNA structures mediate non-autonomous instruction of breast tumour microenvironments bioRxiv 2023-01-19
Henifoff S, Henikoff JG, Kaya-Okur HS et al. Efficient transcription-coupled chromatin accessibility mapping in situ bioRxiv Apr 15 2020 (CHIP-SEQ, Human) CHIP-SEQ Human
Bartosovic M, Kabbe M, Castelo-Branco G et al. Single-cell profiling of histone modifications in the mouse brain bioRxiv 2020-09-02 (Chip Cytometry) Chip Cytometry
Fuglerud BM, Drissler S, Lotto J et al. SOX9 reprograms endothelial cells by altering the chromatin landscape Nucleic acids research 2022-07-29 [PMID: 35904801]
O'Connell TJ, Dadafarin S, Jones M Et al. Androgen Activity Is Associated With PD-L1 Downregulation in Thyroid Cancer Frontiers in cell and developmental biology 2021-08-06 [PMID: 34422798]
Bartosovic M, Kabbe M, Castelo-Branco G Single-cell CUT and Tag profiles histone modifications and transcription factors in complex tissues Nature biotechnology 2021-04-12 [PMID: 33846645]
Madsen JGS, Madsen MS, Rauch A, et al. Highly interconnected enhancer communities control lineage-determining genes in human mesenchymal stem cells Nat Genet 2020-10-06 [PMID: 33020665] (Chip Cytometry) Chip Cytometry
Show All 12 Publications.

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