Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: CHIP-SEQ, ChIP, Chip Cytometry.

Showing Publications 1 - 10 of 17. Show All 17 Publications. Collapse Publications.

Publications using NBP1-72763	Applications	Species
Menon DU, Chakraborty P, Murcia N, Magnuson T. et Al. ARID1A governs the silencing of sex-linked transcription during male meiosis in the mouse Elife 2024-11-26 [PMID: 39589400]
Henifoff S, Henikoff JG, Kaya-Okur HS et al. Efficient transcription-coupled chromatin accessibility mapping in situ bioRxiv Apr 15 2020 (CHIP-SEQ, Human)	CHIP-SEQ	Human
Sanches P Characterization of Cell-Type-Specific and Activity-Dependent Enhancers in Human Neuronal Populations and Their Impact on Neuropsychiatric Disorders Thesis 2021-01-01
Bartosovic M, Kabbe M, Castelo-Branco G et al. Single-cell profiling of histone modifications in the mouse brain bioRxiv 2020-09-02 (Chip Cytometry)	Chip Cytometry
Courret C, Hemmer LW, Wei X et Al. Turnover of retroelements and satellite DNA drives centromere reorganization over short evolutionary timescales in Drosophila PLoS Biol 2024-11-21 [PMID: 39570997]
Simats A, Zhang S, Messerer D, Chong F et Al. Innate immune memory after brain injury drives inflammatory cardiac dysfunction Cell 2024-07-23 [PMID: 39043180]
Harrison Sudholz, Iona S Schuster, Momeneh Foroutan, Xavier Sng, Christopher E Andoniou, Anh Doan, Tania Camilleri, Zihan Shen, Colby Zaph, Mariapia A Degli-Esposti, Nicholas D Huntington, Sebastian Scheer DOT1L maintains NK cell phenotype and function for optimal tumor control. Cell reports 2024-07-01 [PMID: 38865244]
Kawaguchi A, Wang J, Knapp D, Murawala P et Al. A chromatin code for limb segment identity in axolotl limb regeneration Dev Cell 2024-05-24 [PMID: 38788714]
Zhang Y, Zang Y, Chen J et al. A Truncated ETHYLENE INSENSITIVE3/EIN3-Like protein, GhLYI, Regulates Senescence in Cotton Plant physiology 2023-07-11 [PMID: 37430389]
Fuglerud BM, Drissler S, Lotto J et al. SOX9 reprograms endothelial cells by altering the chromatin landscape Nucleic acids research 2022-07-29 [PMID: 35904801]
Zhang B, Srivastava A, Mimitou E et al. Characterizing cellular heterogeneity in chromatin state with scCUT&Tag-pro Nature Biotechnology 2022-08-01 [PMID: 35332340]
Hunold P, Hoehne M, Kiljan M et al. G-quadruplex DNA structures mediate non-autonomous instruction of breast tumour microenvironments bioRxiv 2023-01-19
O'Connell TJ, Dadafarin S, Jones M Et al. Androgen Activity Is Associated With PD-L1 Downregulation in Thyroid Cancer Frontiers in cell and developmental biology 2021-08-06 [PMID: 34422798]
Bartosovic M, Kabbe M, Castelo-Branco G Single-cell CUT and Tag profiles histone modifications and transcription factors in complex tissues Nature biotechnology 2021-04-12 [PMID: 33846645]
Madsen JGS, Madsen MS, Rauch A, et al. Highly interconnected enhancer communities control lineage-determining genes in human mesenchymal stem cells Nat Genet 2020-10-06 [PMID: 33020665] (Chip Cytometry)	Chip Cytometry
Courret C, Hemmer L, Wei X et al. Rapid turnover of centromeric DNA reveals signatures of genetic conflict in Drosophila bioRxiv 2023-08-23 (ChIP) Details: 1:100 ChIP dilution	ChIP
Meijer M, Agirre E, Kabbe M et al. Epigenomic priming of immune genes implicates oligodendroglia in multiple sclerosis susceptibility Neuron 2022-01-28 [PMID: 35093191]
Show All 17 Publications. Collapse Publications.

Array NBP1-72763

• IgG (H+L) Antibodies