Home » CD25/IL-2R alpha » CD25/IL-2R alpha Antibodies » CD25/IL-2R alpha Antibody (PC61.5.3) [Janelia Fluor® 646]
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CD25/IL-2R alpha Antibody (PC61.5.3) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IP, CyTOF-ready
Clone
PC61.5.3
Clonality
Monoclonal
Host
Rat
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB600-710AF350
Alexa Fluor 405
NB600-710AF405
Alexa Fluor 488
NB600-710AF488
Alexa Fluor 532
NB600-710AF532
Alexa Fluor 594
NB600-710AF594
Alexa Fluor 647
NB600-710AF647
Alexa Fluor 700
NB600-710AF700
Alexa Fluor 750
NB600-710AF750
Allophycocyanin
NB600-710APC
Biotin
NB600-710B
DyLight 350
NB600-710UV
DyLight 405
NB600-710V
DyLight 488
NB600-710G
DyLight 550
NB600-710R
DyLight 594
NB600-710DL594
DyLight 650
NB600-710C
DyLight 680
NB600-710FR
DyLight 755
NB600-710IR
FITC
NB600-710F
HRP
NB600-710H
Janelia Fluor 525
NB600-710JF525
Janelia Fluor 549
NB600-710JF549
Janelia Fluor 585
NB600-710JF585
Janelia Fluor 635
NB600-710JF635
Janelia Fluor 669
NB600-710JF669
PE
NB600-710PE
PerCP
NB600-710PCP
Unconjugated
NB600-710
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB600-710
BSA Free
NB600-710
Azide and BSA Free
NBP2-80637
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CD25/IL-2R alpha Antibody (PC61.5.3) [Janelia Fluor® 646] Summary

Immunogen
B6.1 CTL cell line.
Localization
Cell Membrane, Cytoplasm
Specificity
This reacts with the low affinity alpha chain of the IL2 receptor present on activated T and B cells in mice. Clone PC61.5.3 is reported to inhibit IL2 binding and IL2 dependent proliferation.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
IL2RA
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD25/IL-2R alpha Antibody (PC61.5.3) [Janelia Fluor® 646]

  • CD25 antigen
  • CD25
  • IDDM10
  • IL-2 R alpha
  • IL-2 receptor subunit alpha
  • IL2R alpha
  • IL-2R subunit alpha
  • IL2R
  • IL2RA
  • IL-2Ra
  • IL-2-RA
  • IL2-RA
  • interleukin 2 receptor, alpha
  • interleukin-2 receptor subunit alpha
  • p55
  • TAC antigen
  • TCGFR

Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL2RA