Sort By:

Products (31)

page of 3

5'-Nucleotidase/CD73 Antibodies

We offer 5'-Nucleotidase/CD73 Antibodies for use in common research applications: ELISA, Flow Cytometry, Functional, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Simple Western, Western Blot. Each 5'-Nucleotidase/CD73 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our 5'-Nucleotidase/CD73 Antibodies can be used in a variety of model species: Equine, Human, Mouse, Porcine, Rat. Use the list below to choose the 5'-Nucleotidase/CD73 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our 5'-Nucleotidase/CD73 polyclonal antibodies and browse our 5'-Nucleotidase/CD73 monoclonal antibody catalog.

Alternate Names for 5'-Nucleotidase/CD73 Antibodies

anti-5'-Nucleotidase/CD73 antibody, anti-NT5E antibody, anti-5' nucleotidase (CD73) antibody, anti-5'-NT antibody, anti-5'-nucleotidase, ecto (CD73) antibody, anti-CD73 antibody, anti-CD73 antigen antibody, anti-E5NT antibody, anti-EC 3.1.3.5 antibody, anti-ecto-5'-nucleotidase antibody, anti-eN antibody, anti-eNT antibody, anti-NT antibody, anti-NT55'-nucleotidase antibody, anti-NTE antibody, anti-Purine 5-Prime-Nucleotidase antibody

31 Results for "5-nucleotidase-cd73" in Primary Antibodies

List

Grid

NBP2-48480

5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

(1 Review)

(15 Publications)

0.1 ml

NBP2-48480

Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on U87 MG cells with 5'-Nucleotidase/CD73 Antibody (AD2) antibody NBP2-48480 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.

Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.

Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A cell surface stain was performed on U87MG cells with NBP2-48480APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-48480AF350
Alexa Fluor 405	NBP2-48480AF405
Alexa Fluor 488	NBP2-48480AF488
Alexa Fluor 532	NBP2-48480AF532
Alexa Fluor 594	NBP2-48480AF594
Alexa Fluor 647	NBP2-48480AF647
Alexa Fluor 700	NBP2-48480AF700
Alexa Fluor 750	NBP2-48480AF750
Allophycocyanin	NBP2-48480APC
Biotin	NBP2-48480B
CoraFluor 1	NBP2-48480CL1
DyLight 350	NBP2-48480UV
DyLight 405	NBP2-48480V
DyLight 488	NBP2-48480G
DyLight 550	NBP2-48480R
DyLight 594	NBP2-48480DL594
DyLight 650	NBP2-48480C
DyLight 680	NBP2-48480FR
DyLight 755	NBP2-48480IR
FITC	NBP2-48480F
HRP	NBP2-48480H
Janelia Fluor 525	NBP2-48480JF525
Janelia Fluor 549	NBP2-48480JF549
Janelia Fluor 646	NBP2-48480JF646
Janelia Fluor 669	NBP2-48480JF669
PE	NBP2-48480PE
PerCP	NBP2-48480PCP
mFluor Violet 450 SE	NBP2-48480MFV450
mFluor Violet 500 SE	NBP2-48480MFV500
mFluor Violet 610 SE	NBP2-48480MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-48480SS
Azide and BSA Free	NBP2-80552

compare

(select up to 3 products from this page to compare)

NBP1-85740

5'-Nucleotidase/CD73 Antibody

(4 Reviews)

(12 Publications)

0.1 ml

NBP1-85740

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human liver shows strong membranous positivity in hepatocytes.

Immunocytochemistry/Immunofluorescence: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human cell line LHCN-M2 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human aortic valve staining for CD73. Image from verified customer review.

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining for pig aortic valve. Image from verified customer review.

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human endometrium shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human duodenum shows strong positivity in apical membrane in glandular cells.

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.

Reactivity:

Hu, Po, Mu, Rt

Species Glossary

Reactivity: Hu, Po, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

AF4488

5'-Nucleotidase/CD73 Antibody [Unconjugated]

(2 Reviews)

(11 Publications)

100 μg

AF4488

Mouse CD4+ splenocytes was stained with Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Porcine mesenchymal stem cells were stained with Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

5'‑Nucleotidase/CD73 was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to glomeruli and convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

5'-Nucleotidase/CD73 was detected in immersion fixed porcine mesenchymal stem cells using Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder.(a) Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localised to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 μm. (b) Confocal images of Strawberry expression in the liver, spleen, lung, urinary bladder and left and right kidney of ELS infected animals 60 days post left intrarenal injection (top panels) and uninjected control animals (bottom panels). Sections were stained with an antibody against Strawberry (green) and merged images with DAPI (blue) are presented. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26046460), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu, Po

Species Glossary

Reactivity: Mu, Po

Applications:

, , , ,

Applications: , , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF4488

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4488-SP

compare

(select up to 3 products from this page to compare)

AF5795

5'-Nucleotidase/CD73 Antibody [Unconjugated]

(1 Review)

(2 Publications)

100 μg

AF5795

Western blot shows lysates of U266 human myeloma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5795) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for 5'-Nucleotidase/CD73 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

5'-Nucleotidase/CD73 was detected in immersion fixed A431 human epithelial carcinoma cell line using Sheep Anti-Human 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5795) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

5'-Nucleotidase/CD73 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Sheep Anti-Human 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5795) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of U266 human myeloma cell line, loaded at 0.5 mg/mL. A specific band was detected for 5'‑Nucleotidase/CD73 at approximately 86 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human5'‑Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5795) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Simple Western lane view shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, U‑251 MG human glioblastoma cell line, and A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for 5'‑Nucleotidase/CD73 at approximately 86 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human 5'‑Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5795) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Formalin-fixed paraffin-embedded tissue sections of human colon cancer were probed for NT5E mRNA (ACD RNAScope Probe, catalog #437931; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using sheep anti-human NT5E polyclonal antibody (R&D Systems catalog # AF5795) at 1ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5795-SP

compare

(select up to 3 products from this page to compare)

NBP1-60135

5'-Nucleotidase/CD73 Antibody (4G4)

(9 Publications)

0.05 mg

NBP1-60135

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP2-25237

5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

(2 Publications)

0.1 mg

NBP2-25237

CD73 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237) at 1:200 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.

CD73 was detected in immersion fixed paraffin-embedded sections of human cervical cancer using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237) at 1:200 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.

CD73 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237 ) at 1:200 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.

Western Blot: 5'-Nucleotidase/CD73 Antibody (45M4F9) [NBP2-25237] - Analysis of CD73 in a partial recombinant protein using CD73 antibody at 1 ug/ml. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (45M4F9) [NBP2-25237] - Formalin-fixed, paraffin-embedded human breast stained with CD73 antibody (20 ug/ml), peroxidase-conjugate and DAB chromogen.

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6892A

Alt Catalog# IMG-6892A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25243AF350
Alexa Fluor 405	NBP2-25243AF405
Alexa Fluor 488	NBP2-25243AF488
Alexa Fluor 532	NBP2-25243AF532
Alexa Fluor 594	NBP2-25243AF594
Alexa Fluor 647	NBP2-25243AF647
Alexa Fluor 700	NBP2-25243AF700
Alexa Fluor 750	NBP2-25243AF750
Biotin	NBP2-25243B
DyLight 350	NBP2-25243UV
DyLight 405	NBP2-25243V
DyLight 488	NBP2-25243G
DyLight 550	NBP2-25243R
DyLight 594	NBP2-25237DL594
DyLight 650	NBP2-25243C
DyLight 680	NBP2-25243FR
DyLight 755	NBP2-25243IR
FITC	NBP2-25243F
HRP	NBP2-25243H
Janelia Fluor 549	NBP2-25243JF549
Janelia Fluor 646	NBP2-25243JF646

Available Formulations

Formulation	Catalog #	Availability	Price
Azide Free	NBP2-25243
BSA Free	NBP2-25237SS

compare

(select up to 3 products from this page to compare)

NBP2-46259

5'-Nucleotidase/CD73 Antibody (OTI1G2)

(1 Publication)

0.1 ml

NBP2-46259

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-71214AF350
Alexa Fluor 405	NBP2-71214AF405
Alexa Fluor 488	NBP2-71214AF488
Alexa Fluor 532	NBP2-71214AF532
Alexa Fluor 594	NBP2-71214AF594
Alexa Fluor 647	NBP2-71214AF647
Alexa Fluor 700	NBP2-71214AF700
Alexa Fluor 750	NBP2-71214AF750
Allophycocyanin	NBP2-71214APC
Biotin	NBP2-71214B
CoraFluor 1	NBP2-71214CL1
DyLight 350	NBP2-71214UV
DyLight 405	NBP2-71214V
DyLight 488	NBP2-71214G
DyLight 550	NBP2-71214R
DyLight 594	NBP2-71214DL594
DyLight 650	NBP2-71214C
DyLight 680	NBP2-71214FR
DyLight 755	NBP2-71214IR
FITC	NBP2-71214F
HRP	NBP2-71214H
Janelia Fluor 525	NBP2-71214JF525
Janelia Fluor 549	NBP2-71214JF549
Janelia Fluor 646	NBP2-71214JF646
Janelia Fluor 669	NBP2-71214JF669
PE	NBP2-71214PE
PerCP	NBP2-71214PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-71214

compare

(select up to 3 products from this page to compare)

NBP2-15815

5'-Nucleotidase/CD73 Antibody

(1 Publication)

0.1 ml

NBP2-15815

Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 12% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - J5 xenograft, using CD73 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 5'-Nucleotidase/CD73 antibody [N1N3] (NBP2-15815) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

<b>Orthogonal Strategies Validation. </b>Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NBP2-71214

5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free

100 μg

NBP2-71214

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71214] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-71214AF350
Alexa Fluor 405	NBP2-71214AF405
Alexa Fluor 488	NBP2-71214AF488
Alexa Fluor 532	NBP2-71214AF532
Alexa Fluor 594	NBP2-71214AF594
Alexa Fluor 647	NBP2-71214AF647
Alexa Fluor 700	NBP2-71214AF700
Alexa Fluor 750	NBP2-71214AF750
Allophycocyanin	NBP2-71214APC
Biotin	NBP2-71214B
CoraFluor 1	NBP2-71214CL1
DyLight 350	NBP2-71214UV
DyLight 405	NBP2-71214V
DyLight 488	NBP2-71214G
DyLight 550	NBP2-71214R
DyLight 594	NBP2-71214DL594
DyLight 650	NBP2-71214C
DyLight 680	NBP2-71214FR
DyLight 755	NBP2-71214IR
FITC	NBP2-71214F
HRP	NBP2-71214H
Janelia Fluor 525	NBP2-71214JF525
Janelia Fluor 549	NBP2-71214JF549
Janelia Fluor 585	NBP2-71214JF585
Janelia Fluor 635	NBP2-71214JF635
Janelia Fluor 646	NBP2-71214JF646
Janelia Fluor 669	NBP2-71214JF669
PE	NBP2-71214PE
PerCP	NBP2-71214PCP

compare

(select up to 3 products from this page to compare)

MAB57951

5'-Nucleotidase/CD73 Antibody (606117) [Unconjugated]

100 μg

MAB57951

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, Huh-7 human hepatoma cell line, U266 human myeloma cell line, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat 5'-Nucleotidase/CD73 Monoclonal Antibody (Catalog # MAB57951) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 5'-Nucleotidase/CD73 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

5'‑Nucleotidase/CD73 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human/Mouse/Rat 5'‑Nucleotidase/CD73 Monoclonal Antibody (Catalog # MAB57951) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of U‑251 MG human glioblastoma cell line and A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for 5'‑Nucleotidase/CD73 at approximately 86 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat 5'‑Nucleotidase/CD73 Monoclonal Antibody (Catalog # MAB57951) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB57951-SP

compare

(select up to 3 products from this page to compare)

MAB5795

5'-Nucleotidase/CD73 Antibody (606112) [Unconjugated]

(1 Publication)

100 μg

MAB5795

Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Equine 5'-Nucleotidase/CD73 Monoclonal Antibody (Catalog # MAB5795, filled histogram) or isotype control antibody (MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

MDA-MB-231 cells were stained with Mouse Anti-Human/Equine 5'‑Nucleotidase/CD73 Monoclonal Antibody (Catalog # MAB5795, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.

Reactivity:

Hu, Eq

Species Glossary

Reactivity: Hu, Eq

Applications:

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB5795U-100UG
Alexa Fluor 405	FAB5795V-100UG
Alexa Fluor 488	FAB5795G-100UG
Alexa Fluor 532	FAB5795X
Alexa Fluor 594	FAB5795T-100UG
Alexa Fluor 647	FAB5795R-100UG
Alexa Fluor 700	FAB5795N-100UG
Alexa Fluor 750	FAB5795S-100UG
Allophycocyanin	FAB5795A
Allophycocyanin/Cy7	FAB5795APCCY7
Biotin	FAB5795B
CoraFluor 1	FAB5795CL1
DyLight 350	FAB5795D
DyLight 405	FAB5795E
DyLight 488	FAB5795K
DyLight 550	FAB5795L
DyLight 594	FAB5795M
DyLight 650	FAB5795W
DyLight 680	FAB5795Y
DyLight 755	FAB5795Z
FITC	FAB5795F
HRP	FAB5795H
Janelia Fluor 525	FAB5795JF525
Janelia Fluor 549	FAB5795I
Janelia Fluor 646	FAB5795J
Janelia Fluor 669	FAB5795JF669
PE/Atto594	FAB5795PEATT594
PE/Cy5.5	FAB5795PECY55
PE/Cy7	FAB5795PECY7
PerCP	FAB5795C
Phycoerythrin	FAB5795P
mFluor Violet 450 SE	FAB5795MFV450
mFluor Violet 500 SE	FAB5795MFV500
mFluor Violet 610 SE	FAB5795MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB5795A
Carrier Free	FAB5795P
Carrier Free	MAB5795-SP
Carrier Free	FAB5795V-100UG
Carrier Free	FAB5795U-100UG
Carrier Free	FAB5795T-100UG
Carrier Free	FAB5795S-100UG
Carrier Free	FAB5795R-100UG
Carrier Free	FAB5795N-100UG
Carrier Free	FAB5795G-100UG

compare

(select up to 3 products from this page to compare)

NBP2-37271

5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free

0.1 ml

NBP2-37271

Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of A431 cells using NT5E mouse mAb (green) and negative control (red).

Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against human NT5E (AA: 30-250) recombinant protein. (Expected MW is 26.6 kDa)

Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of endometrial cancer tissues using NT5E mouse mAb with DAB staining.

ELISA: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-37271-0.025ml

compare

(select up to 3 products from this page to compare)

page of 3

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

5'-Nucleotidase/CD73 Antibodies

5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

5'-Nucleotidase/CD73 Antibody

5'-Nucleotidase/CD73 Antibody [Unconjugated]

5'-Nucleotidase/CD73 Antibody [Unconjugated]

5'-Nucleotidase/CD73 Antibody (4G4)

5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

5'-Nucleotidase/CD73 Antibody (OTI1G2)

5'-Nucleotidase/CD73 Antibody

5'-Nucleotidase/CD73 Antibody (OTI1G2) - Azide and BSA Free

5'-Nucleotidase/CD73 Antibody (606117) [Unconjugated]

5'-Nucleotidase/CD73 Antibody (606112) [Unconjugated]

5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free