Home » 5'-Nucleotidase/CD73 » 5'-Nucleotidase/CD73 Antibodies » 5'-Nucleotidase/CD73 Antibody (606112) [Phycoerythrin]
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5'-Nucleotidase/CD73 Antibody (606112) [Phycoerythrin]

 (9 Publications)
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Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human 5'-Nucleotidase/CD73 PE-conjugated Monoclonal Antibody (Catalog # FAB5795P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human 5'-Nucleotidase/CD73 PE-conjugated ...read more
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (FAB4867A) and either (A) Mouse Anti-Human 5'-Nucleotidase/CD73 PE-conjugated Monoclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
606112
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB5795U-100UG
Alexa Fluor 405
FAB5795V-100UG
Alexa Fluor 488
FAB5795G-100UG
Alexa Fluor 532
FAB5795X
Alexa Fluor 594
FAB5795T-100UG
Alexa Fluor 647
FAB5795R-100UG
Alexa Fluor 700
FAB5795N-100UG
Alexa Fluor 750
FAB5795S-100UG
Allophycocyanin
FAB5795A
Allophycocyanin/Cy7
FAB5795APCCY7
Biotin
FAB5795B
CoraFluor 1
FAB5795CL1
DyLight 350
FAB5795D
DyLight 405
FAB5795E
DyLight 488
FAB5795K
DyLight 550
FAB5795L
DyLight 594
FAB5795M
DyLight 650
FAB5795W
DyLight 680
FAB5795Y
DyLight 755
FAB5795Z
FITC
FAB5795F
HRP
FAB5795H
Janelia Fluor 525
FAB5795JF525
Janelia Fluor 549
FAB5795I
Janelia Fluor 646
FAB5795J
Janelia Fluor 669
FAB5795JF669
PE/Atto594
FAB5795PEATT594
PE/Cy5.5
FAB5795PECY55
PE/Cy7
FAB5795PECY7
PerCP
FAB5795C
Unconjugated
MAB5795
mFluor Violet 450 SE
FAB5795MFV450
mFluor Violet 500 SE
FAB5795MFV500
mFluor Violet 610 SE
FAB5795MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB5795
Carrier Free
FAB5795A
Carrier Free
MAB5795
Carrier Free
MAB5795-SP
Carrier Free
FAB5795V-100UG
Carrier Free
FAB5795U-100UG
Carrier Free
FAB5795T-100UG
Carrier Free
FAB5795S-100UG
Carrier Free
FAB5795R-100UG
Carrier Free
FAB5795N-100UG
Carrier Free
FAB5795G-100UG
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5'-Nucleotidase/CD73 Antibody (606112) [Phycoerythrin] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human 5'‑Nucleotidase/CD73
Trp27-Ile511
Accession # P21589
Specificity
Detects recombinant human 5'-Nucleotidase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse 5'-Nucleotidase is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NT5E
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
FAB5795P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 5'-Nucleotidase/CD73 Antibody (606112) [Phycoerythrin]

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'-Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • CD73 antigen
  • CD73
  • E5NT
  • EC 3.1.3.5
  • ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase

Background

5'-Nucleotidase (also [ecto]-5'-nucleotidase/5'-NT, designated CD73) is a variably glycosylated, 69-73 kDa member of the 5'-Nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3+ Treg lymphocytes, FDCs and B cells. 5'-Nucleotidase hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human 5'-Nucleotidase is a 523 amino acid (aa) GPI-linked protein (aa 27-549). It contains a large Zn‑dependent nucleotidase catalytic region (aa 28-532) and a C-terminal substrate binding site (aa 500-506). On the cell surface it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa 405-454, and a second possesses a 12 aa substitution for aa 253-574. Over aa 1-511, human 5'‑Nucleotidase shares 88% aa identity with both mouse and rat 5'-Nucleotidase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5'-Nucleotidase/CD73 Antibody (FAB5795P)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(6)
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Human
(7)
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Showing Publications 1 - 9 of 9.
Publications using FAB5795P Applications Species
Abo El-Dahab, MM;El Deen, GN;Aly, RM;Gheith, M; Infrared diode laser enhances human periodontal ligament stem cells behaviour on titanium dental implants Scientific reports 2024-02-20 [PMID: 38378776] (Flow Cytometry, Human) Flow Cytometry Human
A Cataldi, R Amoroso, V di Giacomo, S Zara, C Maccallini, M Gallorini The Inhibition of the Inducible Nitric Oxide Synthase Enhances the DPSC Mineralization under LPS-Induced Inflammation International Journal of Molecular Sciences, 2022-11-23;23(23):. 2022-11-23 [PMID: 36498888] (Human) Human
A Borojevi?, A Jaukovi?, T Kukolj, S Mojsilovi?, H Obradovi?, D Trivanovi?, M Živanovi?, Ž Ze?evi?, M Simi?, B Gobelji?, D Vuji?, D Bugarski Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling Biomolecules, 2022-02-18;12(2):. 2022-02-18 [PMID: 35204824] (Flow Cytometry, Human) Flow Cytometry Human
Marialucia Gallorini, Matthias Widbiller, Carola Bolay, Simone Carradori, Wolfgang Buchalla, Amelia Cataldi et al. Relevance of Cellular Redox Homeostasis for Vital Functions of Human Dental Pulp Cells Antioxidants (Basel) 12/23/2021 [PMID: 35052527]
M Gallorini, R Di Carlo, S Pilato, A Ricci, H Schweikl, A Cataldi, A Fontana, S Zara Liposomes embedded with differentiating factors as a new strategy for enhancing DPSC osteogenic commitment European cells & materials, 2021-01-27;41(0):108-120. 2021-01-27 [PMID: 33501637] (Flow Cytometry, Human) Flow Cytometry Human
Ahmed Othman, Rabab Mubarak, Dina Sabry Fibroblast growth factor-6 enhances CDK2 and MATK expression in microvesicles derived from human stem cells extracted from exfoliated deciduous teeth F1000Research 5/21/2020 [PMID: 32518621]
SJ Li, Y Luo, LM Zhang, W Yang, GG Zhang Targeted introduction and effective expression of hFIX at the AAVS1 locus in mesenchymal stem cells Mol Med Rep, 2017-01-19;0(0):. 2017-01-19 [PMID: 28112377] (Flow Cytometry, Human) Flow Cytometry Human
Christopher J Hewitt Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors Biotechnol J, 2016-02-29;11(4):473-86. 2016-02-29 [PMID: 26632496] (Flow Cytometry, Human) Flow Cytometry Human
Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis. Stem Cells Dev, 2013-12-04;23(5):541-54. 2013-12-04 [PMID: 24172175] (Flow Cytometry, Human) Flow Cytometry Human

Reviews for 5'-Nucleotidase/CD73 Antibody (FAB5795P) (0)

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FAQs for 5'-Nucleotidase/CD73 Antibody (FAB5795P). (Showing 1 - 1 of 1 FAQs).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

Additional 5'-Nucleotidase/CD73 Products

Blogs on 5'-Nucleotidase/CD73.

Check out the latest blog posts on 5'-Nucleotidase/CD73.
Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target
By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu...  Read full blog post.
CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase)
CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble...  Read full blog post.
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Bioinformatics

Gene Symbol NT5E
Uniprot