![Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] diluted at 1:1000.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Western-Blot-NBP2-15815-img0005.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] diluted at 1:1000.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, Func, ICC/IF, IHC, B/N |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human 5'-Nucleotidase/CD73. The exact sequence is proprietary. |
| Localization | Cell membrane; GPI-anchor |
| Predicted Species | Rat (91%), Bovine (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NT5E |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in ICC/IF reported in scientific literature (PMID:33727591). PLA, ICC/IF, Neuratlizing- Assay dependent |
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| Theoretical MW | 63 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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| Publication using NBP2-15815 | Applications | Species |
|---|---|---|
| Petruk N, Tuominen S, Akerfelt M et al. CD73 facilitates EMT progression and promotes lung metastases in triple-negative breast cancer Scientific reports 2021-03-16 [PMID: 33727591] (ICC/IF, Human, Mouse) | ICC/IF | Human, Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for 5'-Nucleotidase/CD73 Antibody (NBP2-15815)Find related products by research area.
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Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu... Read full blog post. |
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CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase) CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble... Read full blog post. |
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![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - CD73 antibody detects CD73 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: CD73 stained by CD73 antibody diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP2-15815-img0006.jpg "Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - CD73 antibody detects CD73 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: CD73 stained by CD73 antibody diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.")
![Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Western-Blot-NBP2-15815-img0004.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 12% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - J5 xenograft, using CD73 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP2-15815-img0003.jpg "Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - J5 xenograft, using CD73 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 5'-Nucleotidase/CD73 antibody [N1N3] (NBP2-15815) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15815_rabbit-polyclonal-5-nucleotidase-cd73-antibody-910202420104432.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 5'-Nucleotidase/CD73 antibody [N1N3] (NBP2-15815) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-15815_rabbit-polyclonal-5-nucleotidase-cd73-antibody-1610202419405713.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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