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5'-Nucleotidase/CD73 Antibody [Unconjugated]

 (11 Publications)
 (2 Reviews)
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Mouse CD4+ splenocytes was stained with Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
Mouse CD4+ splenocytes was stained with Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or control antibody (Catalog # 5‑001‑A, ...read more
Porcine mesenchymal stem cells were stained with Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or isotype control antibody (Catalog # ...read more
5'‑Nucleotidase/CD73 was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 15 µg/mL ...read more
5'-Nucleotidase/CD73 was detected in immersion fixed porcine mesenchymal stem cells using Sheep Anti-Mouse/Porcine 5'-Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 10 µg/mL for 3 ...read more
Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder.(a) Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days ...read more

Product Details

Summary
Reactivity Mu, PoSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF4488
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Catalog# & Formulation Size Price
Carrier Free
AF4488-SP
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5'-Nucleotidase/CD73 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73
Trp29-Lys549
Accession # Q61503
Specificity
Detects mouse 5'‑Nucleotidase/CD73 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
NT5E
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF4488 in the following applications:
Publications
Read Publications using
AF4488 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 5'-Nucleotidase/CD73 Antibody [Unconjugated]

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'-Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • CD73 antigen
  • CD73
  • E5NT
  • EC 3.1.3.5
  • ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase

Background

CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).

  1. Resta, R. et al. (1993) Gene 133:171.
  2. Resta, R. et al. (1998) Immunol. Rev. 161:95.
  3. Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5'-Nucleotidase/CD73 Antibody (AF4488)(11)

We have publications tested in 2 confirmed species: Mouse, Porcine.

We have publications tested in 2 applications: Flow Cytometry, IHC.


Filter By Application
Flow Cytometry
(1)
IHC
(1)
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Mouse
(1)
Porcine
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF4488 Applications Species
Busra Isik, Roman Thaler, Busra B. Goksu, Sabena M. Conley, Hayder Al-Khafaji, Arjunmohan Mohan et al. Hypoxic preconditioning induces epigenetic changes and modifies swine mesenchymal stem cell angiogenesis and senescence in experimental atherosclerotic renal artery stenosis Stem Cell Research & Therapy 2021-12-01 [PMID: 33853680]
Jianying Zhang, Feng Li, Kelly M. Williamson, Susheng Tan, Devon Scott, Kentaro Onishi et al. Characterization of the structure, vascularity, and stem/progenitor cell populations in porcine Achilles tendon (PAT) Cell and Tissue Research 2021-05-01 [PMID: 33496880]
Lise Lefèvre, Jason S. Iacovoni, Hélène Martini, Julie Bellière, Damien Maggiorani, Marianne Dutaur et al. Kidney inflammaging is promoted by CCR2+ macrophages and tissue-derived micro-environmental factors Cellular and Molecular Life Sciences 2021-04-01 [PMID: 33313981]
Yu-Ru V. Shih, Mengqian Liu, Seong Keun Kwon, Masayuki Iida, Ya Gong, Nivedita Sangaj et al. Dysregulation of ectonucleotidase-mediated extracellular adenosine during postmenopausal bone loss Science Advances 2019-08-21 [PMID: 31457100]
Heather M. Perry, Nicole Görldt, Sun-sang J. Sung, Liping Huang, Kinga P. Rudnicka, Iain M. Encarnacion et al. Perivascular CD73+ cells attenuate inflammation and interstitial fibrosis in the kidney microenvironment American Journal of Physiology-Renal Physiology 2019-09-01 [PMID: 31364375]
Samet Bayraktar Molecular and Microarray-based Analysis of Diversity among Resting and Osteogenically Induced Porcine Mesenchymal Stromal Cells of Several Tissue Origin J Tissue Eng Regen Med, 2017-05-19;0(0):. 2017-05-19 [PMID: 27966263] (Flow Cytometry, Porcine) Flow Cytometry Porcine
Kentaro Nishida, Teruyo Kubota, Saki Matsumoto, Junki Kato, Yu Watanabe, Atsuko Yamamoto et al. Expression of Prostatic Acid Phosphatase in Rat Circumvallate Papillae PLOS ONE 2016-06-27 [PMID: 27348306]
Sabrina L. Ena, Jean-François De Backer, Serge N. Schiffmann, Alban de Kerchove d'Exaerde FACS Array Profiling Identifies Ecto-5′ Nucleotidase as a Striatopallidal Neuron-Specific Gene Involved in Striatal-Dependent Learning The Journal of Neuroscience 2013-05-15 [PMID: 23678122]
Robin Dando, Gennady Dvoryanchikov, Elizabeth Pereira, Nirupa Chaudhari, Stephen D. Roper Adenosine Enhances Sweet Taste through A2B Receptors in the Taste Bud The Journal of Neuroscience 2012-01-04 [PMID: 22219293]
Nathaniel A Sowa, Meagen K Voss, Mark J Zylka Recombinant ecto-5'-nucleotidase (CD73) has long lasting antinociceptive effects that are dependent on adenosine A1 receptor activation Mol Pain 2010-04-14 [PMID: 20398264]
Show All 11 Publications.

Reviews for 5'-Nucleotidase/CD73 Antibody (AF4488) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.

Reviews using AF4488:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 IF Other 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23678122
SpeciesOther
  4
reviewed by:
Verified Customer
 IF Mouse 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22219293
SpeciesMouse

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FAQs for 5'-Nucleotidase/CD73 Antibody (AF4488). (Showing 1 - 1 of 1 FAQs).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

Additional 5'-Nucleotidase/CD73 Products

Blogs on 5'-Nucleotidase/CD73.

Check out the latest blog posts on 5'-Nucleotidase/CD73.
Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target
By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu...  Read full blog post.
CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase)
CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Recent Reviews

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5
0
4
2
3
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2
0
1
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 Verified Customer
01/08/2015
Application: IF
Species: Other
read this review
 
 Verified Customer
01/08/2015
Application: IF
Species: Mouse
read this review

Bioinformatics

Gene Symbol NT5E
Uniprot