5'-Nucleotidase/CD73 Antibody (4G4)

The monoclonal antibody 4G4 recognizes both membrane bound and soluble human CD73, also known as ecto-5'-nucleotidase. CD73 is a 70-kDa GPI-anchored cell surface molecule and belongs to the 5'-nucleosidase family. CD73 is useful as marker for lymphocyte differentiation. It is abundantly expressed on the vascular endothelium and at a low level on certain subpopulations of human lymphocytes. Like many glycosyl-phosphatidylinositol (GPI)-anchored molecules, it transmits potent activation signals in T cells when ligated by antibodies. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Ecto-5'-nucleotidase activity is an important mediator of the anti-inflammatory effect by converting extracellular AMP into a potent anti-inflammatory substance adenosine. CD73 has been shown to function as a co-stimulatory molecule in human T cells and to have a role in regulating lymphocyte adhesion. Triggering of CD73 on the surface of lymphocytes, but not on endothelial cells, results in the shedding of the CD73 and increased adhesion of lymphocytes to endothelium via LFA-1 clustering. Furthermore, CD73 has been implicated to mediate homing of skin-infiltrating lymphocytes in vivo. In B-cell chronic lymphocytic leukemia the expression of CD73 is decreased. Besides this, CD73 activity has been implicated as sensitive and useful indicator for mild zinc deficiency. The monoclonal antibody 4G4 causes a reduction in CD73 expression on lymphocytes, reduces enzyme activity, and inhibits the binding of lymphocytes to endothelial cells.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.

Showing Publications 1 - 9 of 9.

Publications using NBP1-60135	Applications	Species
Chisci E, De Giorgi M, Zanfrini E et al. Simultaneous overexpression of human E5NT and ENTPD1 protects porcine endothelial cells against H 2 O 2-induced oxidative stress and cytotoxicity in vitro. Free Radical Biology and Medicine. 2017-04-05 [PMID: 28389406] (ICC/IF, Human)	ICC/IF	Human
Cinti A, De Giorgi M, Chisci E et al. Simultaneous Overexpression of Functional Human HO-1, E5NT and ENTPD1 Protects Murine Fibroblasts against TNF-alpha-Induced Injury In Vitro. PLoS One 2015-01-01 [PMID: 26513260] (ICC/IF, Human)	ICC/IF	Human
Niemela J, Henttinen T, Yegutkin GG, Airas L, Kujari AM, Rajala P, Jalkanen S. IFN-alpha induced adenosine production on the endothelium: a mechanism mediated by CD73 (ecto-5'-nucleotidase) up-regulation. J Immunol;172(3):1646-53. 2004-02-01 [PMID: 14734746]
Airas L, Niemela J, Jalkanen S. CD73 engagement promotes lymphocyte binding to endothelial cells via a lymphocyte function-associated antigen-1-dependent mechanism. J Immunol;165(10):5411-7. 2000-11-15 [PMID: 11067892]
Airas L, Niemela J, Salmi M, Puurunen T, Smith DJ, Jalkanen S. Differential regulation and function of CD73, a glycosyl-phosphatidylinositol-linked 70-kD adhesion molecule, on lymphocytes and endothelial cells. J Cell Biol;136(2):421-31. 1997-01-27 [PMID: 9015312]
Airas L, Salmi M, Jalkanen S. Lymphocyte-vascular adhesion protein-2 is a novel 70-kDa molecule involved in lymphocyte adhesion to vascular endothelium. J Immunol;151(8):4228-38. 1993-10-15 [PMID: 8409398]
Airas L, Hellman J, Salmi M, Bono P, Puurunen T, Smith DJ, Jalkanen S. CD73 is involved in lymphocyte binding to the endothelium: characterization of lymphocyte-vascular adhesion protein 2 identifies it as CD73. J Exp Med;182(5):1603-8. 1995-11-01 [PMID: 7595232]
Cavalcanti BN. Pulp biology: aggression repair. Thesis. 2014-01-01 (WB, Human) Details: 5'-Nucleotidase/CD73 antibody used for WB on lysates of unselected pulp cell populations (uDPC) and selected dental pulp stem cells (sDPC) isolated from a single extracted third molar of human patient (Figure 2B)	WB	Human
De Giorgi M, Cinti A, Pelikant-Malecka I et al. Co-expression of functional human Heme Oxygenase 1, Ecto-5'-Nucleotidase and ecto-nucleoside triphosphate diphosphohydrolase-1 by self-cleaving 2A peptide system. Plasmid. 2015-03-14 [PMID: 25779031] (ICC/IF, Human)	ICC/IF	Human

Array NBP1-60135

• 5'-Nucleotidase/CD73 Antibodies
• 5'-Nucleotidase/CD73 ELISA Kit
• 5'-Nucleotidase/CD73 Kit
• 5'-Nucleotidase/CD73 Lysate
• 5'-Nucleotidase/CD73 Proteins
• 5'-Nucleotidase/CD73 Proteins and Enzymes
• 5'-Nucleotidase/CD73 ELISA