Reactivity | MuSpecies Glossary |
Applications | WB |
Clonality | Polyclonal |
Host | Sheep |
Conjugate | Biotin |
Concentration | LYOPH |
Immunogen | Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73 Trp29-Lys549 Accession # Q61503 |
Specificity | Detects mouse 5'‑Nucleotidase/CD73 in Western blots. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Sheep |
Gene | NT5E |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).
Secondary Antibodies |
Isotype Controls |
Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu... Read full blog post. |
CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase) CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble... Read full blog post. |
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