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5'-Nucleotidase/CD73 Antibody (OTI1G2) [DyLight 550]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI1G2
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71214AF350
Alexa Fluor 405
NBP2-71214AF405
Alexa Fluor 488
NBP2-71214AF488
Alexa Fluor 532
NBP2-71214AF532
Alexa Fluor 594
NBP2-71214AF594
Alexa Fluor 647
NBP2-71214AF647
Alexa Fluor 700
NBP2-71214AF700
Alexa Fluor 750
NBP2-71214AF750
Allophycocyanin
NBP2-71214APC
Biotin
NBP2-71214B
CoraFluor 1
NBP2-71214CL1
DyLight 350
NBP2-71214UV
DyLight 405
NBP2-71214V
DyLight 488
NBP2-71214G
DyLight 594
NBP2-71214DL594
DyLight 650
NBP2-71214C
DyLight 680
NBP2-71214FR
DyLight 755
NBP2-71214IR
FITC
NBP2-71214F
HRP
NBP2-71214H
Janelia Fluor 525
NBP2-71214JF525
Janelia Fluor 549
NBP2-71214JF549
Janelia Fluor 646
NBP2-71214JF646
Janelia Fluor 669
NBP2-71214JF669
PE
NBP2-71214PE
PerCP
NBP2-71214PCP
Unconjugated
NBP2-46259
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46259
Azide and BSA Free
NBP2-71214
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5'-Nucleotidase/CD73 Antibody (OTI1G2) [DyLight 550] Summary

Immunogen
Recombinant protein fragment corresponding to amino acids 119-232 of human 5'-Nucleotidase/CD73 (NP_002517) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NT5E
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for 5'-Nucleotidase/CD73 Antibody (OTI1G2) [DyLight 550]

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'-Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • CD73 antigen
  • CD73
  • E5NT
  • EC 3.1.3.5
  • ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase

Background

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for 5'-Nucleotidase/CD73 Antibody (NBP2-71214R). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

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CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble...  Read full blog post.
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Bioinformatics

Gene Symbol NT5E