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5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

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CD73 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237) at 1:200 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.
CD73 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237) at 1:200 at 37 ° Celsius for 4 minutes. Before ...read more
CD73 was detected in immersion fixed paraffin-embedded sections of human cervical cancer using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237) at 1:200 at 37 ° Celsius for 4 minutes. Before ...read more
CD73 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237 ) at 1:200 at 37 ° Celsius for 4 minutes. Before incubation ...read more
Western Blot: 5'-Nucleotidase/CD73 Antibody (45M4F9) [NBP2-25237] - Analysis of CD73 in a partial recombinant protein using CD73 antibody at 1 ug/ml. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate ...read more
Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (45M4F9) [NBP2-25237] - Formalin-fixed, paraffin-embedded human breast stained with CD73 antibody (20 ug/ml), peroxidase-conjugate and DAB chromogen.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IHC, mIF
Clone
45M4F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-25243AF350
Alexa Fluor 405
NBP2-25243AF405
Alexa Fluor 488
NBP2-25243AF488
Alexa Fluor 532
NBP2-25243AF532
Alexa Fluor 594
NBP2-25243AF594
Alexa Fluor 647
NBP2-25243AF647
Alexa Fluor 700
NBP2-25243AF700
Alexa Fluor 750
NBP2-25243AF750
Biotin
NBP2-25243B
DyLight 350
NBP2-25243UV
DyLight 405
NBP2-25243V
DyLight 488
NBP2-25243G
DyLight 550
NBP2-25243R
DyLight 594
NBP2-25237DL594
DyLight 650
NBP2-25243C
DyLight 680
NBP2-25243FR
DyLight 755
NBP2-25243IR
FITC
NBP2-25243F
HRP
NBP2-25243H
Janelia Fluor 549
NBP2-25243JF549
Janelia Fluor 646
NBP2-25243JF646
View Available Formulations
Catalog# & Formulation Size Price
Azide Free
NBP2-25243
BSA Free
NBP2-25237SS
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5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free Summary

Immunogen
A portion of amino acids 169-324 of human CD73 were used as the immunogen for the antibody.
Specificity
This product can detect Isoform 1 and Isoform of Human CD73.
Predicted Species
Mouse (93%), Rat (92%), Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NT5E
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Multiplex Immunofluorescence 1:200
  • Western Blot 2-5 ug/ml
Publications
Read Publications using
NBP2-25237 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for 5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'-Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • CD73 antigen
  • CD73
  • E5NT
  • EC 3.1.3.5
  • ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase

Background

CD73 is a membrane-bound enzyme having a key role in extracellular ATP metabolism producing adenosine by dephosphorylating AMP. It is highly expressed on endothelial cells but a moderate expression is also observed on a subpopulation of lymphocytes, especially regulatory T cells. Although CD73 on the endothelial cells and lymphocytes are structurally similar, they are differently regulated. Triggering of lymphocyte CD73 with antibodies results in cleavage of CD73 and activation of LFA-1, leading to increased adhesion of lymphocytes to endothelium (Algars A et al., 2011). CD73 plays an important role in carcinogenesis, as adenosine promotes tumor progression and CD73-expressing cancer cell lines are more aggressive (Supernat A et al., 2012). Extracellular adenosine is a potent immunosuppressor that accumulates during tumor growth. Anti-CD73 monoclonal antibody therapy significantly delays primary 4T1.2 and E0771 tumor growth in immune-competent mice and significantly inhibits the development of spontaneous 4T1.2 lung metastases. Antibody therapy is essentially dependent on the induction of adaptive anti-tumor immune responses (Stagg J et al., 2010).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5'-Nucleotidase/CD73 Antibody (NBP2-25237)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IF/IHC.


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FAQs for 5'-Nucleotidase/CD73 Antibody (NBP2-25237). (Showing 1 - 1 of 1 FAQs).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

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Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target
By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu...  Read full blog post.
CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase)
CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble...  Read full blog post.
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Bioinformatics

Gene Symbol NT5E
Uniprot