![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0004.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC, CyTOF-ready |
| Clone | 4G6E3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human 5'-Nucleotidase/CD73 (AA: 30-250) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NT5E |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 63.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for 5'-Nucleotidase/CD73 Antibody (NBP2-37271)Find related products by research area.
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Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu... Read full blog post. |
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CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase) CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NT5E |
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of esophageal cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Immunohistochemistry-NBP2-37271-img0007.jpg "Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of esophageal cancer tissues using NT5E mouse mAb with DAB staining.")
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of A431 cells using NT5E mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Flow-Cytometry-NBP2-37271-img0005.jpg "Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of A431 cells using NT5E mouse mAb (green) and negative control (red).")
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against human NT5E (AA: 30-250) recombinant protein. (Expected MW is 26.6 kDa)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0002.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against human NT5E (AA: 30-250) recombinant protein. (Expected MW is 26.6 kDa)")
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0003.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of endometrial cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Immunohistochemistry-NBP2-37271-img0006.jpg "Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of endometrial cancer tissues using NT5E mouse mAb with DAB staining.")
![ELISA: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-ELISA-NBP2-37271-img0001.jpg "ELISA: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")