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Recombinant Mouse LIF Protein

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Summary
Product Discontinued
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    • Catalog Number
      NBC1-21351
    • Availability
      Product Discontinued

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Recombinant Mouse LIF Protein Summary

Description
A biologically active recombinant protein with a 16 residue His tag and corresponding to 204 total residues of LIF.

Source:E. coli The recombinant protein migrates with an apparent molecular mass of 28-30 kDa in SDS-PAGE.
Preparation
Method
Produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.
Details of Functionality
The bioactivity of NovActive murine LIF was determined by its ability to induce differentiation of murine M1 myeloid leukemia cells. The ED50 value for this effect is typically < 1 ng/ml, corresponding to a specific activity of > 5 x 10e7 U/mg (where 50 U is defined as the amount of mLIF required to induce differentiation of 50% of M1 cells).
Protein/Peptide Type
Recombinant Protein
Gene
LIF
Endotoxin Note
Endotoxin level is less than 0.005ng per ug (0.05EU/ug) as measured by turbidimetric kinetic assay.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Functional
  • Western Blot
Application Notes
NovActiverecombinant mouse LIF is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.
Theoretical MW
22.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse Recombinant protein

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Sterile filtered 0.2 um.PBS, pH 7.2.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute with sterilized water to a final concentration of at least 0.1 mg/ml.

Alternate Names for Recombinant Mouse LIF Protein

  • CDF
  • D Factor
  • DIA
  • differentiation inhibitory activity
  • differentiation stimulating factor
  • Differentiation-stimulating factor
  • Emfilermin
  • HILDA
  • HILDAcholinergic differentiation factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor
  • LIF
  • Melanoma-derived LPL inhibitor
  • MLPLI

Background

LIF (leukemia inhibitory factor, myeloid leukemia inhibitory factor) a pleiotropic lymphoid factor was initially described as a factor that inhibits the proliferation of myeloid leukemia cells and induces their differentiation into macrophages. Other activities of LIF include inhibition of adipogenesis, cholinergic neuron differentiation and bone metabolism. Human and mouse LIF share 78% homology and activities of LIF are not species-specific. Human LIF is active on mouse cells, but murine LIF is not active on human cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Mouse
LIF Protein
NBC1-21351
Recombinant Mouse LIF Protein
Species: Mu
Applications: WB, Func, Bioactivity

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Bioinformatics

Gene Symbol LIF