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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Image from the Alkaline Phosphatase version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72732-img0021.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Image from the Alkaline Phosphatase version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - DyLight 488 conjugated anti-Rabbit IgG was used to demonstrate 2 color STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 ug/mL final concentration of anti-HDAC and detected with 0.2 ug/mL DyLight488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody (colored GREEN). Also shown in this 2-color STED image is a-tubulin monoclonal antibody [MOUSE] detected with ATTO 425 conjugated anti-MOUSE IgG [GOAT] secondary antibody (colored RED). Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72732-img0019.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG. Load: 100 ng per lane. Primary antibody: RABBIT IgG (H&L) Antibody Pre-adsorbed at 1:1,000 for overnight at 4C. Secondary antibody: DyLight 800 goat secondary antibody 1:20,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 and 28 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72732-img0007.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB7575] - Lane 1: Rabbit IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72732-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72732-img0018.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP1-72732-img0026.jpg)
![SDS-Page: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - SDS-Page of Goat anti-Rabbit IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed).Lane 1: Goat anti-Rabbit IgG - Non-Reduced.Lane 2: Goat anti-Rabbit IgG - Reduced.Load: 1.0 ug per lane.Predicted/Observed size: 25 & 55 kDa - Reduced, 160 kDa - Non-Reduced for Goat IgG.Other Band(s): None.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-SDS-Page-NBP1-72732-img0025.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0012.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Image using the DyLight 488 form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Image shows detection of PD-1 in mouse frozen tissue sections (salivary gland injected with human T cells) using DyLight™ 488 conjugated donkey anti-goat IgG (H+L) secondary antibody (green). Image from verified customer review. Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP2-60657-img0007.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): None. Image using the Texas Red form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0001.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Rabbit anti-Transferrin, Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST. Detection shown was using DyLight549 Donkey anti-Rabbit IgG, DyLight 488 Donkey anti-Mouse IgG, and DyLight 649 Donkey anti-Goat IgG at 1:10000 (in blocking buffer min RT). Blots were washed, rinsed in methanol and dried. Image using the DyLight 649 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Image shows anti-Histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was detected using a DyLight™ 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP2-60657-img0006.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP2-60657-img0013.jpg)
![ELISA: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - ELISA Results of Donkey Donkey anti-Goat IgG (H+L) Secondary antibody (Pre-adsorbed) tested against purified Goat IgG MX7. Each well was coated in duplicate with 1.0 ug of Goat IgG as well as Chicken IgG, Guinea Porcine IgG, Hamster IgG, Equine IgG, Mouse IgG, Rabbit IgG, and Rat IgG. The working dilution is 1:56,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Donkey IgG Mx7 HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP2-60657-img0014.jpg)
![Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72793-img0005.jpg "Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.")
![Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - DyLight (R) dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-72793-img0011.jpg)
![Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4C. Block: incubated with blocking buffer for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Predicted/Observed size: 55 kDa for Mouse IgG1.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72793-img0001.jpg)
![Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Mouse IgG1. Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72793-img0002.jpg)
![Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Mouse IgG1. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72793-img0004.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Fluorescence absorption and emission spectra of Dylight 800 in PBS, pH 7.2.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP1-72793-img0006.jpg)
![Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72793-img0008.jpg)
![ELISA: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - ELISA results of purified Rabbit anti-Mouse IgG1 Heavy Chain Secondary antibody tested against purified Mouse IgG1. Each well was coated in duplicate with 1.0 ug of Mouse IgG1. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Goat anti-Rabbit HRP, and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-ELISA-NBP1-72793-img0012.jpg)
![Western Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Western Blot of Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody. Lane 1: Mouse IgG1. Lane 2: Mouse IgG2a. Lane 3: Mouse IgG2b. Lane 4: Mouse IgG3. Lane 5: Mouse IgM. Load: 5 ng per lane.Primary antibody: Mouse IgG2a antibody at 1 ug/mL for overnight at 4C.Secondary antibody: Gt-anti-Rabbit HRP at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 50 kDa and25 kDa, 50 kDa and 25 kDa for Mouse IgG2a. Other band(s): Weak reactivity for to Mouse IgG1.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72794-img0010.jpg "Western Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Western Blot of Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody. Lane 1: Mouse IgG1. Lane 2: Mouse IgG2a. Lane 3: Mouse IgG2b. Lane 4: Mouse IgG3. Lane 5: Mouse IgM. Load: 5 ng per lane.Primary antibody: Mouse IgG2a antibody at 1 ug/mL for overnight at 4C.Secondary antibody: Gt-anti-Rabbit HRP at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 50 kDa and25 kDa, 50 kDa and 25 kDa for Mouse IgG2a. Other band(s): Weak reactivity for to Mouse IgG1.")
![Flow Cytometry: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Antibody [DyLight 405] [NBP1-72921]](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-Flow-Cytometry-NBP1-72794-img0005.jpg)
![Western Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Lane 1: Mouse IgG2a. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG2a Antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4C. Block: incubated with blocking buffer for 30 min at RT. Reaction visualized with alkaline phosphatase substrate for 30 seconds at RT. Predicted/Observed size: 55kDa for Mouse IgG2a.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72794-img0003.jpg)
![Dot Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Mouse IgG2a Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4 Lane 2.5ng](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72794-img0004.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Rabbit anti-Mouse IgG2a Antibody [DyLight 800] [NBP1-72915]](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP1-72794-img0006.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Secondary Antibody [DyLight 488] [NBP1-72891] Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP1-72794-img0008.jpg)
![Dot Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794]](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72794-img0009.jpg)
![Western Blot: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Lane 1: Mouse IgG. Lane 2: None. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase sheep secondary antibody at 1:1,000 for 60 min at RT. Block for30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Image from the HRP version of this antibody.](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6710-img0012.jpg "Western Blot: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Lane 1: Mouse IgG. Lane 2: None. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase sheep secondary antibody at 1:1,000 for 60 min at RT. Block for30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Image from the HRP version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Immunocytochemistry/Immunofluorescence: Sheep anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72924]](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB120-6710-img0009.jpg)
![Western Blot: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein sheep secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6710-img0004.jpg)
![Dot Blot: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Mouse IgG. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Dot-Blot-NB120-6710-img0001.jpg)
![Fluorophore-linked immunosorbent assay: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Fluorophore-linked immunosorbent assay: Sheep anti-Mouse IgG, H/L Chains Antibody [DyLight 405] [NBP1-72929]](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-6710-img0002.jpg)
![ELISA: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - ELISA results of purified Sheep anti-Mouse IgG (H+L) Secondary antibody tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 ug of Mouse IgG. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Dky-anti-Sheep HRP conjugate, and TMB substrate.](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-ELISA-NB120-6710-img0014.jpg)
![Western Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Golden Syrian Hamster IgG (Lane 2) and Armenian Hamster IgG (Lane 3) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 5% Blotto 30 min at 20C, Antibody was used at 1:5000 in blocking buffer for Fluorescent Western blotting. Molecular weight markers are in lane 1.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-71730-img0003.jpg "Western Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Golden Syrian Hamster IgG (Lane 2) and Armenian Hamster IgG (Lane 3) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 5% Blotto 30 min at 20C, Antibody was used at 1:5000 in blocking buffer for Fluorescent Western blotting. Molecular weight markers are in lane 1.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - This image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).. Using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-71730-img0006.jpg)
![Western Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Anti-TNF-alpha was detected using a DyLight 800 conjugate. The image was captured using the Odyssey Infrared Imaging System developed by LI-COR.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-71730-img0002.jpg)
![ELISA: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - ELISA results of purified Goat anti-Hamster IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed) tested against purified Golden Syrian Hamster IgG and Armenian Hamster IgG. Each well was coated in duplicate with 1.0 ug of protein from different species. Golden Syrian Hamster IgG (Green Line), Armenian Hamster IgG (Red Line), Mouse IgG (Blue Line), and Rat IgG (Purple Line). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-71730-img0004.jpg)
![Dot Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Dot Blot for Goat anti-Hamster IgG (H+L) Secondary Antibody [Texas Red] (Pre-adsorbed). Lane 1: 100 ng Hamster IgG. Lanes 2-5: serial dilution 3 fold of Antigen. Primary Antibody: none.Secondary Antibody: Goat anti-Hamster IgG (H+L) Secondary Antibody [Texas Red] (Pre-adsorbed) at 1:1000 dilution at RT for 1 hour. Block: Fluorescent blocking buffer at RT for 30 minutes.. Using the Texas Red format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NBP1-71730-img0005.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP1-71730-img0008.jpg)
![Immunohistochemistry-Paraffin: Lambda Light Chain Antibody [NB100-62139] - Mouse spleen section was stained with Goat Anti-Mouse Lambda-UNLBfollowed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AP Red AP, and hematoxylin.](http://images.novusbio.com/fullsize/Lambda-Light-Chain-Antibody-NB100-62139-img0009.jpg "Immunohistochemistry-Paraffin: Lambda Light Chain Antibody [NB100-62139] - Mouse spleen section was stained with Goat Anti-Mouse Lambda-UNLBfollowed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AP Red AP, and hematoxylin.")
![Flow Cytometry: Lambda Light Chain Antibody [NB100-62139] - Analysis using the TRITC conjugate of NB100-62139. Multiple staining of peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/Lambda-Light-Chain-Antibody-Flow-Cytometry-NB100-62139-img0004.jpg)
![Flow Cytometry: Lambda Light Chain Antibody [NB100-62139] - Analysis of BALB/c splenocytes by multiple stainnig.](http://images.novusbio.com/fullsize/Lambda-Light-Chain-Antibody-Flow-Cytometry-NB100-62139-img0001.jpg)
![Flow Cytometry: Lambda Light Chain Antibody [NB100-62139] - Analysis using the Biotin conjugate of NB100-62139. Staining of Human peripheral blood lymphocytes with goat anti-Human Lambda-PE. and goat anti-Human Kappa-FITC](http://images.novusbio.com/fullsize/Lambda-Light-Chain-Antibody-Flow-Cytometry-NB100-62139-img0002.jpg)
![Flow Cytometry: Lambda Light Chain Antibody [NB100-62139] - Analysis using the PE conjugate of NB100-62139. Multiple staining of BALB/c splenocytes.](http://images.novusbio.com/fullsize/Lambda-Light-Chain-Antibody-Flow-Cytometry-NB100-62139-img0003.jpg)
![Fluorophore-linked immunosorbent assay: Lambda Light Chain Antibody [NB100-62139] - FLISA plate was coated with purified mouse IgG kappa, IgM kappa, IgA kappa, IgG alpha, IgM alpha, and IgA alpha. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Kappa. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/Lambda-Light-Chain-Antibody-Fluorophore-linked-immunosorbent-assay-NB100-62139-img0007.jpg)
![Fluorophore-linked immunosorbent assay: Lambda Light Chain Antibody [NB100-62139] - FLISA plate was coated with purified human IgGk, IgMk, IgAk, IgG alpha, IgM alpha IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-TRITC Image using the TRITC format of this antibody.](http://images.novusbio.com/fullsize/Lambda-Light-Chain-Antibody-Fluorophore-linked-immunosorbent-assay-NB100-62139-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Secondary Antibody [DyLight 488] [NBP1-72873] Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB120-9167-img0001.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Secondary Antibody [DyLight 488] [NBP1-72873] Image from the DyLight 488 version of this antibody.")
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-9167-img0002.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 680 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-9167-img0004.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 800 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-9167-img0006.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-9167-img0007.jpg)
![Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None. Image using the Fluorescein form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6707-img0009.jpg "Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None. Image using the Fluorescein form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - Immunocytochemistry/Immunofluorescence: Donkey anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72930]](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB120-6707-img0011.jpg)
![Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary Antibody: None. Secondary antibody: Mouse IgG (H&L) Antibody Peroxidase Conjugated at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Other Band(s): None. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6707-img0003.jpg)
![Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None. Image using the Texas Red form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6707-img0005.jpg)
![Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - DyLight (R) dyes can be used for two-color western blot detection with low background and high signal. Image using the DyLight 680 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6707-img0008.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-6707-img0016.jpg)
![ELISA: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - ELISA results of purified Donkey anti-Mouse IgG (H+L) Secondary Antibody [Biotin] tested against Mouse IgG. Each well was coated in duplicate with 1.0 ug of Mouse IgG (purple line). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using blocking buffer, Streptavidin HRP conjugate, and TMB substrate.. Using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-ELISA-NB120-6707-img0017.jpg)
![ELISA: Donkey anti-Mouse IgG (H+L) Secondary Antibody [NB120-6707] - ELISA Results of Donkey anti-Mouse IgG (H+L) Secondary antibody tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 ug of Mouse IgG. The working dilution is 1:128,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer , Goat Anti-Donkey HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-ELISA-NB120-6707-img0018.jpg)
![Western Blot: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [NBP1-72795] - Mouse IgG2b. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2b-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72795-img0002.jpg "Western Blot: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [NBP1-72795] - Mouse IgG2b. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.")
![Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [NBP1-72795] - Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG2b Secondary Antibody [DyLight 488] [NBP1-72892] Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2b-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-72795-img0004.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [NBP1-72795] - Rabbit anti-Mouse IgG2b Antibody [DyLight 800] [NBP1-72916]](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2b-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP1-72795-img0003.jpg)
![ELISA: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [NBP1-72795] - ELISA results of purified Rabbit anti-Mouse IgG2b Heavy Chain Secondary antibody tested against purified Mouse IgG2b. Each well was coated in duplicate with 1.0 ug of Mouse IgG2b. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2b-Heavy-Chain-Secondary-Antibody-ELISA-NBP1-72795-img0006.jpg)
![Western Blot: Rabbit anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72731] - Western blot expression of phospho PDH, PDK1, LDH5 and HIF1 alpha in hepatoma HepG2 cells, at 0, 30 and 60 min following exposure to 100 ug/ml of amifostine. Secondary antibody used: Rabbit anti-Mouse IgG (H+L) Secondary Antibody (NBP1-72731). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep30986) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72731-img0007.jpg "Western Blot: Rabbit anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72731] - Western blot expression of phospho PDH, PDK1, LDH5 and HIF1 alpha in hepatoma HepG2 cells, at 0, 30 and 60 min following exposure to 100 ug/ml of amifostine. Secondary antibody used: Rabbit anti-Mouse IgG (H+L) Secondary Antibody (NBP1-72731). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep30986) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72731] - Hep core expression in Huh7 cells. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature. The secondary antibody was diluted in 1% BSA in PBS at 1:500 dilution for 20 minutes at room temperature. Image from verified customer review. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72731-img0004.jpg)
![Western Blot: Rabbit anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72731] - Western Blot: Rabbit anti-Mouse IgG Secondary Antibody [Rhodamine] [NB120-6725] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72731-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72731] - Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72889] Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72731-img0005.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72731]](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP1-72731-img0002.jpg)
![ELISA: Rabbit anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72731] - ELISA results of purified Rabbit anti-Mouse IgG (H+L) Secondary antibody (Pre-adsorbed) tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 ug of Mouse IgG [red line] and Human IgG [blue line]. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin, Goat anti-Rabbit igG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72731-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-Fc-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60664-img0006.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Image from the DyLight 488 version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Analysis of Vero cells. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature. Image from verified customer review. Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-Fc-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60664-img0005.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Image using the DyLight 405 form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60664-img0001.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60664-img0002.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Image using the DyLight 680 form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60664-img0003.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - Image using the DyLight 800 form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60664-img0004.jpg)
![ELISA: Goat anti-Mouse IgG Fc Secondary Antibody [NBP2-60664] - ELISA results of Goat anti-Mouse IgG Fc Secondary antibodyy tested against purified MouseIgG Fc protein. Each well was coated in duplicate with 1.0 ug of MouseIgG Fc. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Donkey anti-Goat IgG Antibody Peroxidase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) and TMB substrate.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-Fc-Secondary-Antibody-ELISA-NBP2-60664-img0009.jpg)