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Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [PE/Cy7]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, DB, ELISA, Flow, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
PE/Cy7

Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [PE/Cy7] Summary

Immunogen
Mouse IgG2a heavy chain
Specificity
Specificity was confirmed by ELISA. For IgG1, IgG2b and IgG3 reactivity was less than 5% against target (IgG2a Heavy Chain, IgG).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Multi-step

Applications/Dilutions

Dilutions
  • Dot Blot
  • ELISA
  • Flow Cytometry
  • Fluorophore-linked immunosorbent assay
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Multi-step

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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