![Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72793-img0005.jpg "Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, DB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum and Mouse IgG. Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. |
| Immunogen | Mouse IgG1 heavy chain |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Multi-step |
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| Application Notes | This product is available in a variety of formats. Anti-Mouse IgG1 Antibody has been tested by ELISA and dot blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Multi-step |
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![Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - DyLight (R) dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-72793-img0011.jpg "Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - DyLight (R) dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. Image using the DyLight 488 format of this antibody.")
![Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4C. Block: incubated with blocking buffer for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Predicted/Observed size: 55 kDa for Mouse IgG1.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72793-img0001.jpg "Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4C. Block: incubated with blocking buffer for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Predicted/Observed size: 55 kDa for Mouse IgG1.")
![Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Mouse IgG1. Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72793-img0002.jpg "Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Mouse IgG1. Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng.")
![Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Mouse IgG1. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72793-img0004.jpg "Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Mouse IgG1. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng.")
![Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Fluorescence absorption and emission spectra of Dylight 800 in PBS, pH 7.2.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP1-72793-img0006.jpg "Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Fluorescence absorption and emission spectra of Dylight 800 in PBS, pH 7.2.")
![Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72793-img0008.jpg "Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]")
![ELISA: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - ELISA results of purified Rabbit anti-Mouse IgG1 Heavy Chain Secondary antibody tested against purified Mouse IgG1. Each well was coated in duplicate with 1.0 ug of Mouse IgG1. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Goat anti-Rabbit HRP, and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-ELISA-NBP1-72793-img0012.jpg "ELISA: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - ELISA results of purified Rabbit anti-Mouse IgG1 Heavy Chain Secondary antibody tested against purified Mouse IgG1. Each well was coated in duplicate with 1.0 ug of Mouse IgG1. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Goat anti-Rabbit HRP, and TMB substrate.")
