![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [DyLight 488] [NBP1-72873] - This image shows anti-histone detection using a DyLight (TM) 488 conjugate (green). Anti-tubulin was detected using a DyLight (TM) 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-[DyLight-488]-Immunocytochemistry-Immunofluorescence-NBP1-72873-img0006.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [DyLight 488] [NBP1-72873] - This image shows anti-histone detection using a DyLight (TM) 488 conjugate (green). Anti-tubulin was detected using a DyLight (TM) 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | DyLight 488 |
| Concentration | LYOPH |
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum |
| Immunogen | Mouse IgM whole molecule |
| Specificity | No reaction was observed against other mouse heavy or light chain proteins. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | IGHM |
| Purity | Multi-step |
| Dilutions |
|
| Application Notes | The emission spectra for this DyLight(TM) conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent). |
![Goat anti-Mouse IgM Heavy Chain Secondary Antibody [DyLight 488]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Goat anti-Mouse IgM Heavy Chain Secondary Antibody [DyLight 488]")
Research Areas for IgM Heavy Chain Antibody (NBP1-72873)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IGHM |
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [DyLight 488] [NBP1-72873] - Comparison of fluorescence adsorption and emission spectra for Dylight 488 and Alexa Fluor 488 in PBS, pH 7.2.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-[DyLight-488]-Fluorophore-linked-immunosorbent-assay-NBP1-72873-img0005.jpg "Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [DyLight 488] [NBP1-72873] - Comparison of fluorescence adsorption and emission spectra for Dylight 488 and Alexa Fluor 488 in PBS, pH 7.2.")
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
