![ELISA: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [HRP] [NBP1-73742] - ELISA Results of Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [HRP] tested against purified Mouse IgGa HRP. Each well was coated in duplicate with 10 ug of Mouse IgG2a. The working dilution is 1:37,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-[HRP]-ELISA-NBP1-73742-img0001.jpg "ELISA: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [HRP] [NBP1-73742] - ELISA Results of Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [HRP] tested against purified Mouse IgGa HRP. Each well was coated in duplicate with 10 ug of Mouse IgG2a. The working dilution is 1:37,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer and TMB substrate.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, DB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | HRP |
| Concentration | LYOPH |
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum |
| Immunogen | Mouse IgG2a heavy chain |
| Specificity | Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or heavy or light chain isotypes. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Multi-step |
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| Application Notes | This product has been tested by ELISA and is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly). |
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| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Gentamicin Sulfate |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 1.0 ml deionized water (or equivalent). |
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