![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) [NB7590] - Western blot of Donkey anti-Goat IgG (H+L) Secondary antibody [PE] (Pre-adsorbed).Lane 1: Goat IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Donkey anti-Goat IgG (H+L) Secondary antibody [PE] (Pre-adsorbed) at 1:1,000 for 60 min at RT.Blocking buffer for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG.Other band(s): None.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-PE-Pre-adsorbed-Western-Blot-NB7590-img0008.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) [NB7590] - Western blot of Donkey anti-Goat IgG (H+L) Secondary antibody [PE] (Pre-adsorbed).Lane 1: Goat IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Donkey anti-Goat IgG (H+L) Secondary antibody [PE] (Pre-adsorbed) at 1:1,000 for 60 min at RT.Blocking buffer for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG.Other band(s): None.")
| Reactivity | GtSpecies Glossary |
| Applications | WB, DB, Flow, ICC/IF |
| Clonality | Polyclonal |
| Host | Donkey |
| Conjugate | PE |
| Format | Pre-adsorbed |
| Description | This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Goat IgG and Goat Serum |
| Immunogen | Goat IgG whole molecule |
| Specificity | This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Donkey |
| Purity | Multi-step |
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| Application Notes | This product has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Specific conditions for reactivity should be optimized by the end user. |
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| Storage | Store at 4C in the dark. Do not freeze. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Purity | Multi-step |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
ICC | Human | 11/25/2014 |
Summary
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Enlarge |
reviewed by:
Verified Customer |
Flow | Human | 11/25/2014 |
Summary
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Research Areas for IgG (H+L) Antibody (NB7590)Find related products by research area.
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| Verified Customer 11/25/2014 |
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| Application: | ICC | |
| Species: | Human |
| Verified Customer 11/25/2014 |
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| Application: | Flow | |
| Species: | Human |
![Dot Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) [NB7590]](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-PE-Pre-adsorbed-Dot-Blot-NB7590-img0006.jpg "Dot Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) [NB7590]")
![Dot Blot of Donkey anti-Goat IgG Antibody Phycoerythrin Conjugated. Antigen: Goat IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: none. Secondary antibody: Donkey anti-Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)at 1:1,000 for 60 min at RT. Block for 1 HR at RT.](http://images.novusbio.com/fullsize/nb7590_donkey-polyclonal-donkey-anti-goat-igg-h-l-secondary-antibody-pe-pre-adsorbed-23520231750500.jpg "Dot Blot of Donkey anti-Goat IgG Antibody Phycoerythrin Conjugated. Antigen: Goat IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: none. Secondary antibody: Donkey anti-Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)at 1:1,000 for 60 min at RT. Block for 1 HR at RT.")
