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Goat anti-Mouse IgM Heavy Chain Secondary Antibody [HRP]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
LYOPH

Goat anti-Mouse IgM Heavy Chain Secondary Antibody [HRP] Summary

Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum
Immunogen
Mouse IgM whole molecule
Specificity
No reaction was observed against other mouse heavy or light chain proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHM
Purity
Multi-step

Applications/Dilutions

Dilutions
  • ELISA 1:30000
  • Immunohistochemistry 1:500 - 1:2000
  • Western Blot 1:1000 - 1:5000
Application Notes
This product is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Readout System

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water (or equivalent).

Alternate Names for Goat anti-Mouse IgM Heavy Chain Secondary Antibody [HRP]

  • Constant region of heavy chain of IgM
  • Ig Mu Chain C Region BOT
  • Ig Mu Chain C Region GAL
  • Ig Mu Chain C Region OU
  • immunoglobulin heavy constant mu
  • Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IGHM