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ZMIZ1/Zimp10 Antibody

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Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ZMIZ1/Zimp10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8107) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZMIZ1/Zimp10 at approximately 120-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ZMIZ1/Zimp10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8107) followed by ...read more
ZMIZ1/Zimp10 was detected in immersion fixed paraffin-embedded sections of human ovary using Sheep Anti-Human ZMIZ1/Zimp10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8107) at 5 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF8107-SP
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ZMIZ1/Zimp10 Antibody Summary

Immunogen
E. coli-derived recombinant human ZMIZ1/Zimp10
Pro878-Ala981
Accession # Q9ULJ6
Specificity
Detects human ZMIZ1/Zimp10 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ZMIZ1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZMIZ1/Zimp10 Antibody

  • FLJ13541
  • hZIMP10
  • KIAA1224
  • KIAA1224Zimp10
  • MIZ
  • PIAS-like protein Zimp10
  • RAI17
  • RAI17TRAFIP10
  • retinoic acid induced 17
  • Retinoic acid-induced protein 17
  • RP11-519K18.1
  • TRAFIP10
  • Zimp10
  • ZIMP10FLJ00092 protein
  • zinc finger MIZ domain-containing protein 1
  • zinc finger, MIZ-type containing 1
  • zinc finger-containing, Miz1, PIAS-like protein on chromosome 10
  • ZMIZ1

Background

Human ZMIZ1 (zinc finger and Miz-domain containing 1), previously called Zimp10 (zinc finger, Miz1, PIAS-like protein on chromosome 10) is a 1067 amino acid (aa), approximately 130 kDa cytoplasmic and nuclear protein. It is a coactivator of the androgen receptor (AR), and enhances AR-mediated transcription and AR sumoylation. It also coactivates other transcription factors such as SMAD4 and p53. It is normally expressed in human ovary, testis and prostate epithelial cells. The ZIMZ1 gene can be fused to ABL1 in a B-cell acute lymphocytic leukemia. Within the region used as an immunogen, human ZMIZ1 shares 98% and 97% aa sequence identity with mouse and rat ZMIZ1, respectively. A 997 aa isoform with an alternate sequence replacing aa 1-94 has been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ZMIZ1
Uniprot